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- The draft genome sequence of the Japanese rhinoceros beetle Trypoxylus dichotomus septentrionalis towards an understanding of horn formation
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Category Archives: Citation
Resetting of 24-nt siRNA landscape is initiated before the first zygotic division in rice
January 2021 Authors: Chenxin Li, Jonathan I. Gent, Hengping Xu, Hong Fu, Scott D. Russell, and Venkatesan Sundaresan Info: In this preprint, the authors report on a study of siRNA profiling in zygotes from the rice plant. They report that … more »
A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol
January 2021 Authors: Dimitrios G Anastasakis, Alexis Jacob, Parthena Konstantinidou, Kazuyuki Meguro, Duncan Claypool, Pavol Cekan, Astrid D Haase, Markus Hafner Info: The authors offer an improvement to the PAR-CLIP method that is more streamlined and does not require the … more »
Posted in Citation
Tagged CLIP, Pipin Prep
Comments Off on A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol
isoCirc catalogs full-length circular RNA isoforms in human transcriptomes
January 2021 Authors: Ruijiao Xin, Yan Gao, Yuan Gao, Robert Wang, Kathryn E. Kadash-Edmondson, Bo Liu, Yadong Wang, Lan Lin & Yi Xing Info: The authors present a protocol, isoCirc, for the full-length sequence determination of circular RNAs. The method … more »
Posted in Citation
Tagged BluePippin, circRNA
Comments Off on isoCirc catalogs full-length circular RNA isoforms in human transcriptomes
Identifying the true number of specimens of the extinct blue antelope (Hippotragus leucophaeus)
Jan 2021 Authors: Elisabeth Hempel, Faysal Bibi, J. Tyler Faith, James S. Brink, Daniela C. Kalthoff, Pepijn Kamminga, Johanna L. A. Paijmans, Michael V. Westbury, Michael Hofreiter & Frank E. Zachos Info: In an effort to better understand the mechanisms … more »
Posted in Citation
Tagged Pippin Prep
Comments Off on Identifying the true number of specimens of the extinct blue antelope (Hippotragus leucophaeus)
ATAC-seq with unique molecular identifiers improves quantification and footprinting
November 2020 Authors: Tao Zhu, Keyan Liao, Rongfang Zhou, Chunjiao Xia & Weibo Xie Info: The authors suggest an improvement on the ATAC-seq method (Assay for Transposase-Accessible Chromatin with high-throughput sequencing). By using unique molecular identifiers to distinguish between transposase … more »
Complete and haplotype-specific sequence assembly of segmental duplication-mediated genome rearrangements using targeted CRISPR-targeted ultra-long read sequencing (CTLR-Seq)
October 2020 Authors: Bo Zhou, GiWon Shin, Stephanie U. Greer, Lisanne Vervoort, Yiling Huang, Reenal Pattni, Marcus Ho, Wing H. Wong, Joris R. Vermeesch, Hanlee P. Ji, Alexander E. Urban Info: In this preprint the authors report on a method, … more »
Posted in Citation
Tagged HLS-CATCH, MinION, oxford nanopore, SageHLS
Comments Off on Complete and haplotype-specific sequence assembly of segmental duplication-mediated genome rearrangements using targeted CRISPR-targeted ultra-long read sequencing (CTLR-Seq)
Ultra-low input single tube linked-read library method enables short-read second-generation sequencing systems to generate highly accurate and economical long-range sequencing information routinely generate highly accurate and economical long-range sequencing information
June 2020 Authors: Zhoutao Chen, Long Pham, Tsai-Chin Wu, Guoya Mo, Yu Xia, Peter L Chang, Devin Porter, Tan Phan, Huu Che, Hao Tran, Vikas Bansal, Justin Shaffer, Pedro Belda-Ferre, Gregory Humphrey, Rob Knight, Pavel Pevzner, Son Pham, Yong Wang … more »
Posted in Citation
Tagged TELL-Seq
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CRISPR–Cas9/long-read sequencing approach to identify cryptic mutations in BRCA1 and other tumour suppressor genes
October 2020 Authors: Tom Walsh, Silvia Casadei, Katherine M Munson, Mary Eng, Jessica B Mandell, Suleyman Gulsuner, Mary-Claire King Info: In this short report, researchers studying young-onset breast cancer used the SageHLS and HLS-CATCH method to purify high molecular weight … more »
A long-read RNA-seq approach to identify novel transcripts of very large genes
July 2020 Authors: Prech Uapinyoying, Jeremy Goecks, Susan M. Knoblach, Karuna Panchapakesan, Carsten G. Bonnemann, Terence A. Partridge, Jyoti K. Jaiswal, and Eric P. Hoffman Info: Scientists researching muscular disorders present a method for studying gene expression of large RNA … more »
Posted in Citation
Tagged BluePippin, iso-seq, PacBio
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Fully phased sequence of a diploid human genome determined de novo from the DNA of a single individual
July 2020 Authors: Ilya Soifer , Nicole L. Fong, Nelda Yi, Andrea T. Ireland, Irene Lam, Matthew Sooknah, Jonathan S. Paw, Paul Peluso, Gregory T. Concepcion, David Rank, Alex R. Hastie, 5 Vladimir Jojic, J. Graham Ruby, David Botstein, Margaret … more »
Posted in Citation
Tagged BluePippin, High Pass, PacBio
Comments Off on Fully phased sequence of a diploid human genome determined de novo from the DNA of a single individual