Author Archives: Alex

ABRF: Tech Lovers Head to Myrtle Beach for Core Lab Meeting

Posted on April 20, 2018 by Alex

Conference season is going strong. Next on our list: the annual meeting of the Association of Biomolecular Resource Facilities in Myrtle Beach, S.C. We never miss this conference — it’s a great event for tech-loving life science people like us. ABRF … more »

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Join Us at the Upcoming Long-Read Workshop at Jackson Lab

Posted on April 19, 2018 by Alex

Long-read sequencing is steadily gaining traction as scientists realize its value for resolving regions of the genome that are intractable with short-read methods. From structural variants to repetitive or GC-rich regions, many clinically important stretches of the human genome just … more »

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Podcast: HMW DNA, Personalized Cancer Vaccines, and Structural Variation

Posted on April 17, 2018 by Alex

There’s a great new podcast interview with Stanford clinical geneticist and oncologist Hanlee Ji about targeting extremely large stretches of DNA by combining CRISPR methods, automated DNA purification with the SageHLS instrument, and 10x Genomics technology. The approach works with … more »

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A Validation Approach of an End-to-End Whole Genome Sequencing Workflow for Source Tracking of Listeria monocytogenes and Salmonella enterica

Posted on April 15, 2018 by Alex

March 2018 Authors: Anne-Catherine Portmann, Coralie Fournier, Johan Gimonet, Catherine Ngom-Bru, et al. Info: Scientists from Nestle Research Center and Nestle Institute of Health Sciences aimed to establish guidelines for whole-genome sequencing used in contamination investigations in the food industry. … more »

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Full-length mRNA sequencing uncovers a widespread coupling between transcription initiation and mRNA processing

Posted on April 12, 2018 by Alex

March 2018 Authors: Seyed Yahya Anvar, Guy Allard, Elizabeth Tseng, Gloria Sheynkman, et al. Info: Researchers from Leiden University Medical Center and other institutions studied the relationships between transcription initiation and splicing and polyadenylation events in breast cancer cells and … more »

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Transmission of microRNA antimiRs to mouse offspring via the maternal-placental-fetal unit

Posted on April 10, 2018 by Alex

March 2018 Authors: Jonas Hönig, Ivana Mižíková, Claudio Nardiello, David Emanuel Surate Solaligue, et al. Info: In Germany, researchers delivered microRNA inhibitors to pregnant mice, finding that the antimiRs were then detectable in offspring, where they caused fewer off-target effects … more »

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New Protocol: Analyze Small Extracellular RNAs with PippinHT

Posted on April 3, 2018 by Alex

Good protocols are the currency of any lab. If your research involves working with small RNAs, be sure to check out this carefully documented protocol published in Extracellular RNA, part of Springer Nature’s Methods in Molecular Biology book series. The protocol … more »

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Expert Q&A: Winston Timp on New Challenges in Sample Prep

Posted on March 27, 2018 by Alex

We’re learning as we go: that’s the message from Winston Timp, assistant professor at Johns Hopkins University, about how labs are handling the new demands placed on sample prep techniques by ever-changing sequencing technologies. Timp’s impressive results, particularly with handling … more »

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Cost-Effective Microbiome Sequencing with 10x Genomics and Athena Assembler

Posted on March 21, 2018 by Alex

If you missed Ami Bhatt’s talk at AGBT last month, a bioRxiv preprint is a great way to catch up on her team’s impressive work characterizing microbial communities — from the human gut to the sea floor. Bhatt and her … more »

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Improving saliva shotgun metagenomics by chemical host DNA depletion

Posted on March 12, 2018 by Alex

February 2018 Authors: Clarisse Marotz, Jon Sanders, Cristal Zuniga, Livia Zaramela, Rob Knight, and Karsten Zengler Info: Scientists from the University of California, San Diego, developed and evaluated a new protocol for enriching microbial DNA collected from fresh and frozen … more »

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