Monthly Archives: April 2018

Assembly and annotation of a draft genome sequence for Glycine latifolia, a perennial wild relative of soybean

Posted on April 29, 2018 by Alex

April 2018 Authors: Qiong Liu, Sungyul Chang, Glen Hartman, and Leslie Domier Info: At the University of Illinois, scientists sequenced a wild perennial relative of the soybean plant, producing a 939 Mb draft assembly using linked reads from a single … more »

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Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

Posted on April 29, 2018 by Alex

April 2018 Authors: Paola Gaiero, Hana Šimková, JanVrána, Federico Santiñaque, et al. Info: Scientists from Wageningen University & Research and other institutions embarked on a project to improve sample prep protocols for obtaining high molecular weight DNA from plant cells … more »

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ABRF: Tech Lovers Head to Myrtle Beach for Core Lab Meeting

Posted on April 20, 2018 by Alex

Conference season is going strong. Next on our list: the annual meeting of the Association of Biomolecular Resource Facilities in Myrtle Beach, S.C. We never miss this conference — it’s a great event for tech-loving life science people like us. ABRF … more »

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Join Us at the Upcoming Long-Read Workshop at Jackson Lab

Posted on April 19, 2018 by Alex

Long-read sequencing is steadily gaining traction as scientists realize its value for resolving regions of the genome that are intractable with short-read methods. From structural variants to repetitive or GC-rich regions, many clinically important stretches of the human genome just … more »

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Podcast: HMW DNA, Personalized Cancer Vaccines, and Structural Variation

Posted on April 17, 2018 by Alex

There’s a great new podcast interview with Stanford clinical geneticist and oncologist Hanlee Ji about targeting extremely large stretches of DNA by combining CRISPR methods, automated DNA purification with the SageHLS instrument, and 10x Genomics technology. The approach works with … more »

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A Validation Approach of an End-to-End Whole Genome Sequencing Workflow for Source Tracking of Listeria monocytogenes and Salmonella enterica

Posted on April 15, 2018 by Alex

March 2018 Authors: Anne-Catherine Portmann, Coralie Fournier, Johan Gimonet, Catherine Ngom-Bru, et al. Info: Scientists from Nestle Research Center and Nestle Institute of Health Sciences aimed to establish guidelines for whole-genome sequencing used in contamination investigations in the food industry. … more »

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Full-length mRNA sequencing uncovers a widespread coupling between transcription initiation and mRNA processing

Posted on April 12, 2018 by Alex

March 2018 Authors: Seyed Yahya Anvar, Guy Allard, Elizabeth Tseng, Gloria Sheynkman, et al. Info: Researchers from Leiden University Medical Center and other institutions studied the relationships between transcription initiation and splicing and polyadenylation events in breast cancer cells and … more »

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Transmission of microRNA antimiRs to mouse offspring via the maternal-placental-fetal unit

Posted on April 10, 2018 by Alex

March 2018 Authors: Jonas Hönig, Ivana Mižíková, Claudio Nardiello, David Emanuel Surate Solaligue, et al. Info: In Germany, researchers delivered microRNA inhibitors to pregnant mice, finding that the antimiRs were then detectable in offspring, where they caused fewer off-target effects … more »

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New Protocol: Analyze Small Extracellular RNAs with PippinHT

Posted on April 3, 2018 by Alex

Good protocols are the currency of any lab. If your research involves working with small RNAs, be sure to check out this carefully documented protocol published in Extracellular RNA, part of Springer Nature’s Methods in Molecular Biology book series. The protocol … more »

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