Tag Archives: Pippin Prep

Broad Institute Teams with Sage Science for Automated Sizing

Posted on June 20, 2012 by Alex

Don’t miss this great blog post from the Broad Institute (“A Sage partnership”) describing collaborative work between their genome sequencing team and Sage Science to design a better size selection process for the Broad’s sequencing pipeline. Headed up by Sheila … more »

Posted in Blog | Tagged , , | Comments Off on Broad Institute Teams with Sage Science for Automated Sizing

Automated Size Selection Key for RADseq: PLoS One Publication

Posted on June 11, 2012 by Alex

Congratulations to Sage Science customer Hopi Hoekstra and her team at Harvard University for their recent publication in PLoS One! Dr. Hoekstra, who works in the Organismic & Evolutionary Biology and the Molecular & Cellular Biology departments at Harvard, reports … more »

Posted in Blog | Tagged , | Comments Off on Automated Size Selection Key for RADseq: PLoS One Publication

Double Digest RADseq: An Inexpensive Method for De Novo SNP Discovery and Genotyping in Model and Non-Model Species

Posted on May 23, 2012 by Alex

May 2012 Authors: Brant K. Peterson, Jesse N. Weber, Emily H. Kay, Heidi S. Fisher, Hopi E. Hoekstra Info: An NGS method that allows inexpensive HT genotyping at moderate depth without prior knowledge of any markers. A high impact paper. … more »

Posted in Citation | Tagged , , , | Comments Off on Double Digest RADseq: An Inexpensive Method for De Novo SNP Discovery and Genotyping in Model and Non-Model Species

At Emory, Size Selection Saves Time for NGS Prep

Posted on May 17, 2012 by Alex

At the genome center at Emory University, scientists credit the Pippin Prep with shaving almost a full day off the sample prep process for Illumina’s mate-pair library prep. Chad Haase, laboratory manager at the Emory GRA Genome Center, says that … more »

Posted in Blog | Tagged , , , | Comments Off on At Emory, Size Selection Saves Time for NGS Prep

New Cassettes Use Internal Standards

Posted on April 27, 2012 by Alex

We’ve just released new dye-free cassettes for the BluePippin and Pippin Prep that use dye-labeled DNA markers* as internal standards, instead of typical external marker set. In the new product, the labeled markers will come premixed wi th the Ficoll … more »

Posted in Blog, Tips and Applications | Tagged , , , | 2 Comments

“Overflow Detection” definition on the Pippin Prep to be eliminated

Posted on April 17, 2012 by Alex

| | Pippin Prep users are likely familiar with our “overflow detection” cassette definitions that are found in the “Cassette Type” drop down menu of the Protocol Editor. We use these definitions because Pippin cassettes exhibit a phenomenon where the … more »

Posted in Blog, Tips and Applications | Tagged , | Comments Off on “Overflow Detection” definition on the Pippin Prep to be eliminated

Low Incidence of DNA Sequence Variation in Human Induced Pluripotent Stem Cells Generated by Nonintegrating Plasmid Expression

Posted on March 23, 2012 by Alex

March 2012 Authors: Linzhao Cheng, Nancy F. Hansen, Ling Zhao, Yutao Du, Chunlin Zou, Frank X. Donovan, Bin-Kuan Chou, Guangyu Zhou, Shijie Li, Sarah N. Dowey, Zhaohui Ye, NISC Comparative Sequencing Program, Settara C. Chandrasekharappa, Huanming Yang, James C. Mullikin, … more »

Posted in Citation | Tagged , , , , | Comments Off on Low Incidence of DNA Sequence Variation in Human Induced Pluripotent Stem Cells Generated by Nonintegrating Plasmid Expression

Mutation discovery in mice by whole exome sequencing

Posted on December 23, 2011 by Alex

December 2011 Authors: Heather Fairfield, Griffith J Gilbert, Mary Barter, Rebecca R Corrigan, Michelle Curtain, Yueming Ding, Mark D’Ascenzo, Daniel J Gerhardt, Chao He, Wenhui Huang, Todd Richmond, Lucy Rowe, Frank J Probst, David E Bergstrom, Stephen A Murray, Carol … more »

Posted in Citation | Tagged , , , , | Comments Off on Mutation discovery in mice by whole exome sequencing

Sage Science Pippin Prep To Be Commercialized Through Life Technologies For Ion PGM™ Sequencer Customers

Posted on October 11, 2011 by Alex

Beverly, MA—October 11, 2011—Sage Science, Inc., a privately-held, MA-based company, today announced that it has signed an agreement with Life Technologies Corporation (NASDAQ:LIFE) enabling Life Technologies to offer the Pippin Prep DNA size selection system to users of the Ion … more »

Posted in Press Release | Tagged , | Comments Off on Sage Science Pippin Prep To Be Commercialized Through Life Technologies For Ion PGM™ Sequencer Customers

Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn

Posted on September 1, 2011 by Alex

September 2011 Authors: Laura G. Reinholdt, Yueming Ding, Griffith T. Gilbert, Anne Czechanski, Jeffrey P. Solzak, Randall J. Roper, Mark T. Johnson, Leah Rae Donahue, Cathleen Lutz, Muriel T. Davisson Info: Illumina PE libraries were constructed and size selected at … more »

Posted in Citation | Tagged , , , | Comments Off on Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn