Double Digest RADseq: An Inexpensive Method for De Novo SNP Discovery and Genotyping in Model and Non-Model Species

May 2012

Brant K. Peterson, Jesse N. Weber, Emily H. Kay, Heidi S. Fisher, Hopi E. Hoekstra

An NGS method that allows inexpensive HT genotyping at moderate depth without prior knowledge of any markers. A high impact paper. Extremely reproducible size selection is crucial to the method and the authors used Pippin Prep for this purpose.

PLoS ONE 7(5): e37135. doi:10.1371/journal.pone.0037135

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