Tag Archives: Pippin Prep

Crop Science Collaboration Uses Next-Gen Sequencing with Pippin Prep

Posted on November 12, 2012 by Alex

A collaboration among scientists at Monsanto and Bayer Crop Science has demonstrated the use of next-generation sequencing and a new bioinformatics method for analyzing the genome sequence of crops that have been genetically modified. Lead authors David Kovalic and Carl … more »

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Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation

Posted on November 1, 2012 by Alex

November 2012 Authors: Michael A. Quail, Yong Gu, Harold Swerdlow, Matthew Mayho Info: An evaluation of semi-automated size selection systems for use in size-selection for NGS libraries at the Sanger Centre. A comparison to manual gel purification and magnetic beads. … more »

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Cell Paper Offers New Understanding of Myc Mechanism in Cancer

Posted on October 30, 2012 by Alex

A big paper came out of Richard Young’s lab at the Whitehead Institute last month in Cell describing the mechanism through which the c-Myc transcription factor causes elevated expression in tumor cells. We were happy to see that the Pippin … more »

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Cell Paper Reports Massive Genome Study for Lung Cancer

Posted on October 15, 2012 by Alex

A paper that came out last month in Cell describes a massive effort to generate the exome and/or genome sequences of 183 tumor/normal pairs for people with a specific type of lung cancer. Authors hailed from Harvard University, New York … more »

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The Use of NexGen Sequencing and Junction Sequence Analysis Bioinformatics to Achieve Molecular Characterization of Crops Improved Through Modern Biotechnology

Posted on October 15, 2012 by Alex

October 2012 Authors: David Kovalic, Carl Garnaat, Liang Guo, Yongpan Yan, Jeanna Groat, Andre Silvanovich, Lyle Ralston, Mingya Huang, Qing Tian, Allen Christian, Nordine Cheikh, Jerry Hjelle, Stephen Padgette and Gary Bannon Info: Materials and Methods: Illumina TruSeq paired-end library … more »

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Draft Genome Sequence of Flavobacterium sp. Strain F52, Isolated from the Rhizosphere of Bell Pepper (Capsicum annuum L. cv. Maccabi)

Posted on October 1, 2012 by Alex

October 2012 Authors: Max Kolton, Stefan J. Green, Yael Meller Harel, Noa Sela, Yigal Elad, and Eddie Cytryna Info: A brief report of whole genome sequence of a novel Flavobacterium. The bacterial library was created with the EpiCentre Nextera kit. … more »

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Towards quantitative metagenomics of wild viruses and other ultra-low concentration DNA samples: a rigorous assessment and optimization of the linker amplification method

Posted on September 29, 2012 by Alex

September 2012 Authors: Melissa B. Duhaime, Li Deng, Bonnie T. Poulos, and Matthew B. Sullivan Info: A study of most reproducible and sample-efficient methods for sequencing meta-genomic samples with very low abundance samples (20 L ocean water ->1 pg to … more »

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Scientists Use Pippin in Sequencing Effort for Seven Cholera Isolates

Posted on September 24, 2012 by Alex

A paper published this month in BMC Genomics discusses findings from a collaborative project to sequence cholera samples linked to the 2010 outbreak in Haiti — and we’re pleased to report that the Pippin platform from Sage Science was used … more »

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High depth, whole-genome sequencing of cholera isolates from Haiti and the Dominican Republic

Posted on September 23, 2012 by Alex

September 2012 Authors: Rachel Sealfon, Stephen Gire, Crystal Ellis, Stephen Calderwood, Firdausi Qadri, Lisa Hensley, Manolis Kellis, Edward T Ryan, Regina C LaRocque, Jason B Harris, Pardis C Sabeti Info: Study performs high-depth NGS on V. cholerae strains from Haiti … more »

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Mapping the Hallmarks of Lung Adenocarcinoma with Massively Parallel Sequencing

Posted on September 15, 2012 by Alex

September 2012 Authors: Marcin Imielinski et al. Info: A landmark study of lung cancer genomes with 183 tumor/normal genomes compared. Sequencing was carried out mainly by the Broad Illumina Platform (8 Pippins in production). The Pippin Prep is cited in … more »

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