Tag Archives: Pippin Prep

Multiplex target capture with double-stranded DNA probes

Posted on May 23, 2013 by Alex

May 2013 Authors: Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe Info: The authors describe a new method for targeted NGS using double-stranded probes rather than single-stranded ones, improving sensitivity for variant detection, and at … more »

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RESTseq – Efficient Benchtop Population Genomics with RESTriction Fragment SEQuencing

Posted on May 10, 2013 by Alex

May 2013 Authors: Eckart Stolle1, Robin F. A. Moritz Info: The authors present a new method for SNP analysis in population genomics. The Pippin Prep was used to size select DNA for the Ion Torrent PGM platform . Citation: PLoS … more »

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FOXO1 regulates expression of a microRNA cluster on X chromosome

Posted on May 2, 2013 by Alex

May 2013 Authors: Ruchi Singhal, Jonathan E. Bard, Norma J. Nowak, Michael J.Buck, and Eugene S. Kandel Info: The Pippin Prep was used in size selection with the Illumina TruSeq Small RNA sequencing protocol. Citation: AGING 5(5): 347-356 http://www.impactaging.com/papers/v5/n5/full/100558.html

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Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data

Posted on April 23, 2013 by Alex

April 2013 Authors: Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson Info: The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification … more »

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Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae

Posted on April 10, 2013 by Alex

April 2013 Authors: Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen Info: The Pippin Prep was used to size-select Illumina RNA-seq libraries. Citation: Int. J. Mol. Sci. 2013, 14, 9080-9098 http://dx.doi.org/10.3390/ijms14059080

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NHGRI Scientists Find Transgenic DNA Has More Rearrangements than Expected

Posted on April 8, 2013 by Alex

Amanda DuBose didn’t set out to come up with a new technical solution to characterizing transgenic DNA in animal models. A postdoctoral fellow in Francis Collins’ lab at the National Human Genome Research Institute, DuBose was using mice to study … more »

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H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis

Posted on March 30, 2013 by Alex

March/April 2013 Authors: Kyle C. Costa, Thomas J. Lie, Michael A. Jacobs, John A. Leigh Info: The authors constructed deletions in several key metabolism genes to study the mechanism of H2 independent growth in this hydrogenotropic strain. Several mutants were … more »

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At Iowa Core Lab, Pippin Sizing Is Essential for MicroRNA Studies

Posted on March 21, 2013 by Alex

Kevin Knudtson’s DNA Facility at the University of Iowa acquired a Pippin Prep almost a year ago, and in the time since, the automated size selection tool has become an integral part of the core lab’s microRNA pipeline. “Pippin is … more »

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Pippin Shines in Size Selection Comparison from Sanger

Posted on March 13, 2013 by Alex

A paper that came out December in the journal Electrophoresis compares Pippin Prep size selection with Caliper’s Labchip XT and finds that Pippin gives “very tight size distributions” not seen with other sizing methods. “Evaluation and optimisation of preparative semi-automated … more »

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At MIT, Pippin Enables Splice Variant Detection and MicroRNA Analysis

Posted on February 26, 2013 by Alex

At the BioMicro Center at MIT, Director Stuart Levine, PhD, recently introduced the Pippin Prep from Sage Science to enable key applications — including splice variant analysis with RNA-seq and microRNA analysis — that were not possible on other platforms. … more »

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