Kyle C. Costa, Thomas J. Lie, Michael A. Jacobs, John A. Leigh
The authors constructed deletions in several key metabolism genes to study the mechanism of H2 independent growth in this hydrogenotropic strain. Several mutants were found that shed new light on alternative mechanisms of methanogenic growth. The mutants were mapped by whole genome sequencing, in which Pippin Prep was used to size select the libraries.
mBio (4)2:00062-13 March/April 2013