Tag Archives: PacBio

Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing Approach for In-depth Understanding of Genes in Secondary Metabolism Pathways of Camellia sinensis

Posted on July 24, 2017 by Alex

July 2017 Authors: Qingshan Xu, Junyan Zhu, Shiqi Zhao, Yan Hou, et al. Info: Scientists from Anhui Agricultural University in China performed long-read transcriptome sequencing of the tea plant Camellia sinensis to better understand its production of flavonoids, theanine, and … more »

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PacBio Users: Size Selection Is Essential for Generating Excellent Results

Posted on July 6, 2017 by Alex

The Sage Science team was delighted to attend and co-sponsor PacBio’s annual East Coast user group meeting in Baltimore last week, particularly since there was a half-day session devoted to our favorite subject: sample prep. There were plenty of customer … more »

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Long-Read Isoform Sequencing Reveals a Hidden Complexity of the Transcriptional Landscape of Herpes Simplex Virus Type 1

Posted on June 12, 2017 by Alex

June 2017 Authors: Dóra Tombácz, Zsolt Csabai, Attila Szűcs, Zsolt Balázs, et al. Info: Scientists from Hungary and California conducted isoform sequencing with PacBio to analyze the transcriptome of herpes simplex virus type 1. They discovered dozens of previously unidentified … more »

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Comprehensive comparison of Pacific Biosciences and Oxford Nanopore Technologies and their applications to transcriptome analysis

Posted on June 10, 2017 by Alex

June 2017 Authors: Jason Weirather, Mariateresa de Cesare, Yunhao Wang, Paolo Piazza, Vittorio Sebastiano, Xiu-Jie Wang, David Buck, Kin Fai Au Info: In this F1000 publication, scientists describe an evaluation of long-read sequencing technologies, finding that PacBio provides better data … more »

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Comparative Analysis of Ralstonia solanacearum Methylomes

Posted on May 10, 2017 by Alex

April 2017 Authors: Ivan Erill, Marina Puigvert, Ludovic Legrand, Rodrigo Guarischi-Sousa, et al. Info: Scientists report results of analyzing epigenetic patterns in two strains of a plant pathogen. They determined that methylation regulates virulence and adaptation to different host plants. … more »

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Tripartite species interaction: eukaryotic hosts suffer more from phage susceptible than from phage resistant bacteria

Posted on May 10, 2017 by Alex

April 2017 Authors: Carolin Wendling, Agnes Piecyk, Dominik Refardt, Cynthia Chibani, et al. Info: Scientists investigated the extent to which a bacterium’s susceptibility or resistance to prophages alters its own effect as a pathogen, finding that bacteria that were more … more »

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Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere

Posted on April 25, 2017 by Alex

March 2017 Authors: Henk J. Schouten, Henri vande Geest, Sofia Papadimitriou, Marian Bemer, Jan G. Schaart, Marinus J. M. Smulders, Gabino Sanchez Perez, Elio Schijlen Info: Scientists from Wageningen University and Research sequenced and analyzed several Arabidopsis thaliana plants to … more »

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Plasmodium malariae and P. ovale genomes provide insights into malaria parasite evolution

Posted on March 25, 2017 by Alex

February 2017 Authors: Gavin G. Rutledge, Ulrike Böhme, Mandy Sanders, Adam J. Reid, et al. Info: In this paper, scientists generated a new reference genome and a manually curated draft assembly for two Plasmodium species. This effort made it possible … more »

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Two stable variants of Burkholderia pseudomallei strain MSHR5848 express broadly divergent in vitro phenotypes associated with their virulence differences

Posted on March 25, 2017 by Alex

February 2017 Authors: A. A. Shea, R. C. Bernhards, C. K. Cote, C. J. Chase, et al. Info: Scientists from the United States Army Medical Research Institute of Infectious Diseases compared two strains of Burkholderia pseudomallei to find variants that … more »

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De Novo Genome and Transcriptome Assembly of the Canadian Beaver (Castor canadensis)

Posted on March 25, 2017 by Alex

February 2017 Authors: Si Lok, Tara A. Paton, Zhuozhi Wang, Gaganjot Kaur, et al. Info: Researchers in Toronto report a high-quality draft assembly of the 2.7 Gb beaver genome using SMRT Sequencing. They include an analysis of gene content, such … more »

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