Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere

March 2017

Henk J. Schouten, Henri vande Geest, Sofia Papadimitriou, Marian Bemer, Jan G. Schaart, Marinus J. M. Smulders, Gabino Sanchez Perez, Elio Schijlen

Scientists from Wageningen University and Research sequenced and analyzed several Arabidopsis thaliana plants to understand the mutagenic effects of Agrobacterium tumefaciens. As part of this project, they identified a 50 bp partial T-DNA insert and named it a T-DNA splinter. BluePippin was used to remove short fragments from the library prior to sequencing with PacBio.

Plant Cell Reports, volume 36, Issue 3, pp 493–504
DOI: 10.1007/s00299-017-2098-z

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