Tag Archives: MiSeq

Dual redundant sequencing strategy: Full-length gene characterisation of 1056 novel and confirmatory HLA alleles

Posted on July 25, 2017 by Alex

August 2017 Authors: Albrecht V, Zweiniger C, Surendranath V, Lang K, et al. Info: Scientists in Germany describe a high-throughput approach for characterizing novel HLA alleles. The method involves sequencing the full gene with PacBio and MiSeq. For PacBio sequencing, … more »

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The Conserved RNA Binding Cyclophilin, Rct1, Regulates Small RNA Biogenesis and Splicing Independent of Heterochromatin Assembly

Posted on June 25, 2017 by Alex

June 2017 Authors: An-Yun Chang, Stephane Castel, Evan Ernst, Hyun Soo Kim, Robert Martienssen Info: Cold Spring Harbor Laboratory researchers studied the Rct1 protein in S. pombe and found that it is involved in processing pericentromeric transcripts into siRNAs. For … more »

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Creighton Lab Boosts Yield, Sequencing Efficiency with BluePippin

Posted on June 13, 2017 by Alex

At Creighton University in Omaha, Neb., Dr. Anna Selmecki’s lab explores various fungal species to understand genome instability, pathogenesis, and the acquisition of drug resistance. For these investigations, her team relies heavily on whole genome sequencing, using both the Illumina … more »

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Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia

Posted on February 25, 2017 by Alex

January 2017 Authors: Jonathan A. Shortt, Daren C. Card, Drew R. Schield, Yang Liu, Bo Zhong, Todd A. Castoe, Elizabeth J. Carlton, and David D. Pollock Info: Researchers used ddRAD-seq to study the schistosome species S. japonicum miracidia, finding that … more »

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QseC inhibition as an antivirulence approach for colitis-associated bacteria

Posted on February 6, 2017 by Alex

January 2017 Authors: Michelle G. Rooks, Patrick Veiga, Analise Z. Reeves, Sydney Lavoie, et al. Info: In this paper, scientists from Harvard and other institutions describe a study of quorum-sensing regulators in E. coli as a possible means of reducing … more »

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Susceptibility to Aminoglycosides and Distribution of aph and aac(3)-XI Genes among Corynebacterium striatum Clinical Isolates

Posted on January 6, 2017 by Alex

December 2016 Authors: Jesús Navas, Marta Fernández-Martínez, Carlos Salas, María Eliecer Cano, Luis Martínez-Martínez Info: Scientists in Spain analyzed several strains of the pathogen Corynebacterium striatum and found that many were resistant to drugs most commonly used to treat these … more »

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Generation of Full-Length Class I Human Leukocyte Antigen Gene Consensus Sequences for Novel Allele Characterization

Posted on December 9, 2016 by Alex

December 2016 Authors: Peter M. Clark, Jamie L. Duke, Deborah Ferriola, Valia Bravo-Egana, Tunde Vago, Aniqa Hassan, Anna Papazoglou, and Dimitri Monos Info: Scientists from The Children’s Hospital of Philadelphia evaluated an NGS-based HLA typing kit from Omixon. They analyzed … more »

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A draft genome of the brown alga, Cladosiphon okamuranus, S-strain: a platform for future studies of ‘mozuku’ biology

Posted on September 20, 2016 by Alex

August 2016 Authors: Koki Nishitsuji, Asuka Arimoto, Kenji Iwai, Yusuke Sudo, Kanako Hisata, Manabu Fujie, Nana Arakaki, Tetsuo Kushiro, Teruko Konishi, Chuya Shinzato, Noriyuki Satoh and Eiichi Shoguchi Info: Scientists from Japan sequenced the genome of Cladosiphon okamuranus, an edible … more »

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Circulating Extracellular RNA Markers of Liver Regeneration

Posted on August 19, 2016 by Alex

July 2016 Authors: Irene K. Yan, Xue Wang, Yan W. Asmann, Hiroaki Haga, Tushar Patel Info: Mayo Clinic researchers performed partial hepatectomies in mice and then monitored RNA activity in serum samples to find a signature associated with liver regeneration. … more »

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Double Pippin for Optimized RAD-seq

Posted on July 13, 2016 by Alex

Scientists in China and the UK recently published an open-access optimized protocol for RAD-seq in the Theoretical and Applied Genetics journal. The method is targeted at large studies of plants and enables users to specify sequence coverage parameters. From lead … more »

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