Tag Archives: DNA Sequencing

A Hybrid Genetic Linkage Map of Two Ecologically and Morphologically Divergent Midas Cichlid Fishes(Amphilophus spp.) Obtained by Massively Parallel DNA Sequencing (ddRADSeq)

Posted on January 1, 2013 by Alex

January 2013 Authors: Hans Recknagel, Kathryn R. Elmer, and Axel Meyer Info: RADseq was used to develop linkage maps for two highly divergent Cichlid fish. Pippin Prep was used to size select the libraries. Citation: G3 (Bethesda) 3(1):65-74 January 2013 … more »

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Holiday Greetings from Sage Science

Posted on December 28, 2012 by Alex

It’s that time of year again — we slow down at work, do some reflection, and gear up for a great (lucky?) ‘13…. In the field of genomics, 2012 was a fascinating year. On the NGS front, placements of desktop … more »

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IL-10 Produced by Induced Regulatory T Cells (iTregs) Controls Colitis and Pathogenic Ex-iTregs during Immunotherapy

Posted on December 10, 2012 by Alex

December 2012 Authors: Erica G. Schmitt, Dipica Haribhai, Jason B. Williams, Praful Aggarwal, Shuang Jia, Louis-Marie Charbonnier, Ke Yan, Rachel Lorier, et al. Info: The study included sequencing of the T-cell receptor. For this sequencing, the authors used Ion Torrent … more »

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Bactobolin Resistance is Conferred by Mutations in the L2 Ribosomal Protein

Posted on December 1, 2012 by Alex

December 2012 Authors: Josephine Chandler, Thao T. Truong, Patricia M. Silva, Mohammad R. Seyedsayamdost, Galvin Carr, Matthew Radey, Michael A. Jacobs, Elizabeth H. Sims, Jon Clardy, E. Peter Greenberg Info: Authors were searching for cause of resistance to antibiotic Bactobolin. … more »

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SATB1-mediated functional packaging of chromatin into loops

Posted on November 23, 2012 by Alex

November 2012 Authors: Terumi Kohwi-Shigematsu, Yoshinori Kohwi, Keiko Takahashi, Hunter W. Richards, Stephen D. Ayers, Hye-Jung Han, Shutao Cai Info: Among other things, this paper outlines methods for mapping all DNA binding sites for a global chromatin organizing protein, SATB1. … more »

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For Low-Input DNA Work, Scientists Recommend Pippin for Best Size Selection

Posted on November 20, 2012 by Alex

Scientists at the University of Arizona, led by senior author and newly named Moore Foundation Investigator Matthew Sullivan, have published details of how to optimize sample prep methods for next-gen sequencing projects in which input DNA is negligible. As part … more »

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Use of Four Next-Generation Sequencing Platforms to Determine HIV-1 Coreceptor Tropism

Posted on November 18, 2012 by Alex

November 2012 Authors: John Archer, Jan Weber, Kenneth Henry, Dane Winner, Richard Gibson, Lawrence Lee, Ellen Paxinos, Eric J. Arts, David L. Robertson, Larry Mimms, Miguel E. Quinones-Mateu Info: Comparative study of four NGS platforms (454, Illumina, PacBio, Ion) in … more »

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Crop Science Collaboration Uses Next-Gen Sequencing with Pippin Prep

Posted on November 12, 2012 by Alex

A collaboration among scientists at Monsanto and Bayer Crop Science has demonstrated the use of next-generation sequencing and a new bioinformatics method for analyzing the genome sequence of crops that have been genetically modified. Lead authors David Kovalic and Carl … more »

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At ASHG, Interest Soars for Advances in Sequencing Technologies

Posted on November 9, 2012 by Alex

We were thrilled to escape the apocalyptic weather of the northeast for a few days to attend the American Society of Human Genetics annual meeting in San Francisco. It’s one of our favorite meetings, and with nearly 7,000 scientists registered, … more »

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Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation

Posted on November 1, 2012 by Alex

November 2012 Authors: Michael A. Quail, Yong Gu, Harold Swerdlow, Matthew Mayho Info: An evaluation of semi-automated size selection systems for use in size-selection for NGS libraries at the Sanger Centre. A comparison to manual gel purification and magnetic beads. … more »

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