Tag Archives: BluePippin

Churning Out New Microbial Genome Sequences

Posted on August 25, 2015 by Alex

PacBio users have been regularly serving up new microbial genome assemblies, and we’re glad to see that they’re using our BluePippin automated DNA size selection instrument to get the best results. These are just some of the genome announcements published … more »

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Scientists Optimize Mate-Pair Sequencing with SageELF

Posted on July 10, 2015 by Alex

In a Biotechniques paper this month, scientists from The Genome Analysis Centre describe a new method for mate-pair sequencing that saves time and money while decreasing the amount of input DNA required. The method is based on SageELF, which automatically … more »

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Assembly and diploid architecture of an individual human genome via single-molecule technologies

Posted on July 1, 2015 by Alex

June 2015 Authors: Matthew Pendleton, Robert Sebra, Andy Wing Chun Pang, Ajay Ummat, Oscar Franzen, Tobias Rausch, Adrian M Stütz, William Stedman, Thomas Anantharaman, Alex Hastie, Heng Dai, Markus Hsi-Yang Fritz, Han Cao, Ariella Cohain, Gintaras Deikus, Russell E Durrett, … more »

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Hybrid Single-Molecule Approach Produces Highly Contiguous Diploid Human Genome Assembly

Posted on July 1, 2015 by Alex

A team of scientists from the Icahn School of Medicine at Mount Sinai, Weill Cornell Medical College, Cold Spring Harbor Laboratory, European Molecular Biology Laboratory, and other institutions published the first analysis of a diploid human genome produced by combining … more »

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Learning About Long Reads: The PacBio User Group Meeting

Posted on June 15, 2015 by Alex

This week we’re traveling to Baltimore for the annual east coast user group meeting for Pacific Biosciences customers. We’re a sponsor of the event and look forward to the great scientific presentations these meetings have become known for. Click here … more »

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Modified Mate-Pair Protocol Lowers Cost, Boosts Library Yield

Posted on June 11, 2015 by Alex

Regular readers of the Sage Science blog know that we can never resist a good methods paper. We enjoyed this publication from the phyloinformatics group at RIKEN detailing a modified protocol for generating high-quality mate-pair libraries while significantly reducing costs. … more »

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Limitations of Climatic Data for Inferring Species Boundaries: Insights from Speckled Rattlesnakes

Posted on June 5, 2015 by Alex

June 2015 Authors: Jesse M. Meik, Jeffrey W. Streicher, A. Michelle Lawing, Oscar Flores-Villela, and Matthew K. Fujita Info: In this paper, scientists in Texas and Mexico challenge conventional climate-based species boundaries in the speckled rattlesnake, using genomic and other … more »

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Fecal virome analysis of three carnivores reveals a novel nodavirus and multiple gemycircularviruses

Posted on June 4, 2015 by Alex

May 2015 Authors: Nádia Conceição-Neto, Mark Zeller, Elisabeth Heylen, Hanne Lefrère, João Rodrigo Mesquita and Jelle Matthijnssens Info: This publication in Virology Journal describes metagenomics analyses of fecal samples from badger, mongoose, and otter to better understand viral populations in … more »

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Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencing

Posted on June 3, 2015 by Alex

May 2015 Authors: Kaori Tatsumi, Osamu Nishimura, Kazu Itomi, Chiharu Tanegashima, and Shigehiro Kuraku Info: Scientists from RIKEN modified the recommended protocol for the Nextera Mate Pair Sample Prep Kit from Illumina to improve library yield and quality while minimizing … more »

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Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies

Posted on May 14, 2015 by Alex

April 2015 Authors: Sagar M Utturkar, Dawn M Klingeman, José M Bruno-Barcena, Mari S Chinn, Amy M Grunden, Michael Köpke & Steven D Brown Info: Researchers from the University of Tennessee, North Carolina State University, and other organizations present data … more »

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