Tag Archives: Amplicon

Ingestible Osmotic Pill for In‐vivo Sampling of Gut Microbiome

Posted on July 30, 2019 by Alex

July 2019 Authors: Hojatollah Rezaei Nejad, Bruno C. M. Oliveira, Aydin Sadeqi, Amin Dehkharghani, Ivanela Kondova Jan A. M. Langermans, Jeffrey S. Guasto, Saul Tzipori, Giovanni Widmer, Sameer R. Sonkusale Info: From Engineering and Nano Labs at Tufts University, and … more »

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In Skin Microbiome Study, Scientists Find Host Genotype Influences Bacterial Populations

Posted on August 29, 2013 by Alex

A paper that came out in the Journal of Innate Immunity this summer reports that a person’s genotype affects which microbes will colonize his or her skin, which in turn may alter that person’s defense mechanisms against pathogenic organisms. “Skin … more »

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Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses

Posted on June 12, 2013 by Alex

Authors: Sanne P. Smeekens, c Curtis Huttenhower, Anca Riza, Frank L. van de Veerdonk, Patrick L.J.M. Zeeuwen, Joost Schalkwijk, Jos W.M. van der Meer, Ramnik J. Xavier, Mihai G. Netea, Dirk Gevers Info: Genomic DNA was subjected to 16S PCR … more »

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Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data

Posted on April 23, 2013 by Alex

April 2013 Authors: Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson Info: The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification … more »

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Tag Team: At DFCI, Pippin and Nextera Make Better Libraries

Posted on January 18, 2013 by Alex

Over at the Dana-Farber Cancer Institute, scientists have been doing some really interesting work pairing the Pippin Prep with Illumina’s Nextera kit for library preparation. Zach Herbert, associate director of the Molecular Biology Core Facilites at DFCI, says that he … more »

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Next-Generation Sequencing for Rodent Barcoding: Species Identification from Fresh, Degraded and Environmental Samples

Posted on November 16, 2012 by Alex

November 2012 Authors: Maxime Galan, Marie Pages, Jean-Francois Cosson Info: Genotyping rodent strains using targeted NGS of informative regions of cytochrome b and cytochrome c oxidase 1 genes. PCR fragments ~250 bp in length were generated (with barcoded primers) from … more »

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