Next-Generation Sequencing for Rodent Barcoding: Species Identification from Fresh, Degraded and Environmental Samples

November 2012

Maxime Galan, Marie Pages, Jean-Francois Cosson

Genotyping rodent strains using targeted NGS of informative regions of cytochrome b and cytochrome c oxidase 1 genes. PCR fragments ~250 bp in length were generated (with barcoded primers) from a variety of sample types, pooled, and size selected as a pool on Pippin Prep to remove non-specific PCR products.

PLoS ONE 7(11): e48374. doi:10.1371/journal.pone.0048374

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