Genome-wide DNA methylation profiling using the methylation-dependent restriction enzyme LpnPI

December 2017

Ruben Boers, Joachim Boers, Bas de Hoon, Christel Kockx, et al.


Scientists at Erasmus Medical Center determined that analysis of genome-wide methylation is challenging when fragment sizes are too small and that better epigenetic results are possible with MeD-seq. The enzyme LpnPI, they found, “is blocked by a fragment size smaller than 32 bp.” The team used PippinHT to purify PCR products prior to sequencing on the Illumina HiSeq 2500.

Genome Research

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