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Recent Posts
- Cost-effective and Flexible Preimplantation Genetic Testing (PGT) Using Adaptive Sampling-based Targeted Nanopore Sequencing (ASTN-seq)
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- Leveraging the power of long reads for targeted sequencing
- Resolving the 22q11.2 deletion using CTLR-Seq reveals chromosomal rearrangement mechanisms and individual variance in breakpoints
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Author Archives: Alex
HLS-CATCH and TELL-Seq™: a new route to Targeted Long-Fragment Linked-Read Sequencing
At the recent 2020 AGBT meeting Universal Sequencing gave a data-filled poster on their new Transposase Enzyme Linked Long-read Sequencing kit and workflow, known as TELL-Seq™. The technology features a unique combination of bead-linked and soluble transposases that can generate … more »
Posted in Blog
Tagged HLS-CATCH, linked-read, SageHLS, TELL-Seq
Comments Off on HLS-CATCH and TELL-Seq™: a new route to Targeted Long-Fragment Linked-Read Sequencing
SageHLS Helps Unravel the Neuropsychiatric 22q11.2 Deletion Region1q11
An international collaboration led by the Stanford labs of Alexander Urban and Hanlee Ji, used the SageHLS system to map a Mb-sized deletion in a complex region of the human genome, 22q11.2, that is associated with a variety of neurodevelopmental … more »
Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis
February 2020 Authors: Phuong-Uyen C. Dinh, Dipti Paudel, Hayden Brochu, Kristen D. Popowski, M. Cyndell Gracieux, Jhon Cores, Ke Huang, M. Taylor Hensley, Erin Harrell, Adam C. Vandergriff, Arianna K. George, Raina T. Barrio, Shiqi Hu, Tyler A. Allen, Kevin … more »
Posted in Citation
Tagged microRNA, Pippin Prep
Comments Off on Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis
A spontaneous complex structural variant in rcan-1 increases exploratory behavior and laboratory fitness of Caenorhabditis elegans
February 2020 Authors: Yuehui Zhao, Lijiang Long, Jason Wan, Shweta Biliya, Shannon C. Brady, Daehan Lee, Akinade Ojemakinde, Erik C. Andersen, Fredrik O. Vannberg, Hang Lu, Patrick T. McGrath Info: Researchers studying genetic adaptive changes using C. elegans recombinant inbred … more »
Posted in Citation
Tagged BluePippin, oxford nanopore
Comments Off on A spontaneous complex structural variant in rcan-1 increases exploratory behavior and laboratory fitness of Caenorhabditis elegans
Amplification-free long read sequencing reveals unforeseen CRISPR-Cas9 off-target activity
February 2020 Authors: Ida Höijer, Josefin Johansson, Sanna Gudmundsson, Chen-Shan Chin, Ignas Bunikis, Susana Häggqvist, Anastasia Emmanouilidou, Maria Wilbe, Marcel den Hoed, Marie-Louise Bondeson, Lars Feuk, Ulf Gyllensten, Adam Ameur Info: In this preprint, the authors propose a method for … more »
Capture of complete ciliate chromosomes in single sequencing reads reveals widespread chromosome isoforms
December 2019 Authors: Kelsi A. Lindblad, Jananan S. Pathmanathan, Sandrine Moreira, John R. Bracht, Robert P. Sebra, Elizabeth R. Hutton & Laura F. Landweber Info: Researchers sequenced the whole genome of Oxytricha trifallax, a model protozoan ciliate, using long read … more »
Posted in Citation
Tagged BluePippin, PacBio
Comments Off on Capture of complete ciliate chromosomes in single sequencing reads reveals widespread chromosome isoforms
SageHLS sample prep for ultra-long Nanopore Sequencing at NextOmics/GrandOmics
NextOmics/GrandOmics is the largest third-generation sequencing service company in China. The NextOmics division focuses on animal, plant and bacterial genome sequencing, while GrandOmics focuses on human genome sequencing. GrandOmics became the first certified PromethIon service provider in China in June, … more »
Posted in Uncategorized
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Comparison of mitochondrial DNA variants detection using short- and long-read sequencing
August 2019 Authors: Ahmed N. Alkanaq, Kohei Hamanaka, Futoshi Sekiguchi, Masataka Taguri, Atsushi Takata, Noriko Miyake, Satoko Miyatake, Takeshi Mizuguchi, Naomichi Matsumot Info: The genotypes of mitochondrial DNA variants are compared using both short-read (Illumina Hi-SeqX10) and long-read (PacBio Sequel) … more »
Posted in Citation
Tagged BluePippin, mtDNA, PacBio
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Ingestible Osmotic Pill for In‐vivo Sampling of Gut Microbiome
July 2019 Authors: Hojatollah Rezaei Nejad, Bruno C. M. Oliveira, Aydin Sadeqi, Amin Dehkharghani, Ivanela Kondova Jan A. M. Langermans, Jeffrey S. Guasto, Saul Tzipori, Giovanni Widmer, Sameer R. Sonkusale Info: From Engineering and Nano Labs at Tufts University, and … more »
Posted in Citation
Tagged 16S, Amplicon, Illumina, Pippin Prep
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Distinct Profiles of Cell-Free MicroRNAs in Plasma of Veterans with Post-Traumatic Stress Disorder
July 2019 Authors: Min Young Lee, David Baxter, Kelsey Scherler , Taek-Kyun Kim, Xiaogang Wu , Duna Abu-Amara, Janine Flory, Rachel Yehuda, Charles Marma, Marti Jett, Inyoul Lee, Kai Wang, and Leroy Hood Info: Researchers report on studies that profile … more »