A spontaneous complex structural variant in rcan-1 increases exploratory behavior and laboratory fitness of Caenorhabditis elegans

February 2020

Yuehui Zhao, Lijiang Long, Jason Wan, Shweta Biliya, Shannon C. Brady, Daehan Lee, Akinade Ojemakinde, Erik C. Andersen, Fredrik O. Vannberg, Hang Lu, Patrick T. McGrath

Researchers studying genetic adaptive changes using C. elegans recombinant inbred lines (RIL). A single RIL with a high fitness and exploration phenotype was observed that did not share these traits relative to the parental strains. Short-read sequencing indicated a spontaneous complex rearrangement of the rcan-1 gene. This rearrangement was resolved into five unique tandem inversion/duplications using Oxford Nanopore long-read sequencing. The authors suggest that colonization of new environments can create evolutionary pressure to produce complex rearrangements that can act to modify gene expression in ways besides increased gene dosage.

BluePippin was used to size select 10-50kb DNA fragments for Oxford Nanopore MinION library preparation.

Author Affiliations:
Georgia Institute of Technology, Atlanta, Georgia
Northwestern University, Evanston, Illinois

PLOS Genetics
DOI: 10.1371/journal.pgen.1008606

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