November 2020
Authors:
Tao Zhu, Keyan Liao, Rongfang Zhou, Chunjiao Xia & Weibo Xie
Info:
The authors suggest an improvement on the ATAC-seq method (Assay for Transposase-Accessible Chromatin with high-throughput sequencing). By using unique molecular identifiers to distinguish between transposase insertions and PCR duplicates. They go on to demonstrate the UMI-ATAC-seq method more accurately quantifies chromatin accessibility and improve the sensitivity of identifying transcription footprints.
The PippinHT was used to size select libraries prior to sequencing.
Author Affiliations:
Huazhong Agricultural University, Wuhan China
Citation:
Nature Communications Biology
DOI: 10.1038/s42003-020-01403-4