ATAC-seq with unique molecular identifiers improves quantification and footprinting

November 2020

Tao Zhu, Keyan Liao, Rongfang Zhou, Chunjiao Xia & Weibo Xie

The authors suggest an improvement on the ATAC-seq method (Assay for Transposase-Accessible Chromatin with high-throughput sequencing). By using unique molecular identifiers to distinguish between transposase insertions and PCR duplicates. They go on to demonstrate the UMI-ATAC-seq method more accurately quantifies chromatin accessibility and improve the sensitivity of identifying transcription footprints.

The PippinHT was used to size select libraries prior to sequencing.

Author Affiliations:
Huazhong Agricultural University, Wuhan China

Nature Communications Biology
DOI: 10.1038/s42003-020-01403-4

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