-
Recent Posts
- Leveraging the power of long reads for targeted sequencing
- Resolving the 22q11.2 deletion using CTLR-Seq reveals chromosomal rearrangement mechanisms and individual variance in breakpoints
- CTLR-Seq Protocol
- Processing frozen archival human DNA samples for large-scale SQK-LSK114 Oxford Nanopore long-read DNA sequencing SOP v1
- Identifying the best PCR enzyme for library amplification in NGS
Tags
10x Genomics ABRF agbt Amplicon ASHG ASMS BluePippin ChIP-Seq ddRADseq DNA Sequencing Featured genotyping High Pass HiSeq HLA HLS HLS-CATCH Illumina ILMN Ion Torrent isoseq Mate-pair Sequencing Methods microRNA miRNA miRNA isolation MiSeq ONT oxford nanopore PacBio PAG Paired-end Sequencing PippinHT Pippin Prep Pippin System podcast Protein Mass Spec Protein Size Fractionation proteomics RAD-seq RNA-seq RNA sequencing SageELF SageHLS Tips and ApplicationsRecent Comments
- Alex on And Now, a Word from Our Customer Service Department
- Donna on And Now, a Word from Our Customer Service Department
- Tony Gill on And Now, a Word from Our Customer Service Department
- Alex on And Now, a Word from Our Customer Service Department
- Andrea on And Now, a Word from Our Customer Service Department
Archives
- December 2024
- July 2024
- June 2024
- April 2024
- February 2024
- January 2024
- December 2023
- August 2023
- June 2023
- March 2023
- February 2023
- January 2023
- November 2022
- September 2022
- April 2022
- January 2022
- September 2021
- July 2021
- June 2021
- May 2021
- February 2021
- January 2021
- November 2020
- October 2020
- July 2020
- May 2020
- April 2020
- March 2020
- February 2020
- January 2020
- November 2019
- August 2019
- July 2019
- June 2019
- May 2019
- April 2019
- March 2019
- February 2019
- January 2019
- December 2018
- October 2018
- September 2018
- May 2018
- April 2018
- March 2018
- February 2018
- January 2018
- December 2017
- November 2017
- October 2017
- September 2017
- August 2017
- July 2017
- June 2017
- May 2017
- April 2017
- March 2017
- February 2017
- January 2017
- December 2016
- November 2016
- October 2016
- September 2016
- August 2016
- July 2016
- June 2016
- May 2016
- April 2016
- March 2016
- February 2016
- January 2016
- December 2015
- November 2015
- October 2015
- September 2015
- August 2015
- July 2015
- June 2015
- May 2015
- April 2015
- March 2015
- February 2015
- January 2015
- December 2014
- November 2014
- October 2014
- September 2014
- August 2014
- July 2014
- June 2014
- May 2014
- April 2014
- March 2014
- February 2014
- January 2014
- December 2013
- November 2013
- October 2013
- September 2013
- August 2013
- July 2013
- June 2013
- May 2013
- April 2013
- March 2013
- February 2013
- January 2013
- December 2012
- November 2012
- October 2012
- September 2012
- August 2012
- July 2012
- June 2012
- May 2012
- April 2012
- March 2012
- February 2012
- December 2011
- October 2011
- September 2011
- June 2011
- February 2011
- November 2010
- October 2010
- August 2010
Monthly Archives: July 2016
Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing
June 2016 Authors: Shruthi Sridhar Vembar, Matthew Seetin, Christine Lambert, Maria Nattestad, Michael C. Schatz, Primo Baybayan, Artur Scherf, and Melissa Laird Smith Info: Scientists in France and the US used PacBio sequencing to characterize the AT-rich genome of this … more »
Posted in Citation
Tagged BluePippin, High Pass, Pippin Pulse
Comments Off on Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing
A survey of the sorghum transcriptome using single-molecule long reads
June 2016 Authors: Salah E. Abdel-Ghany, Michael Hamilton, Jennifer L. Jacobi, Peter Ngam, Nicholas Devitt, Faye Schilkey, Asa Ben-Hur & Anireddy S. N. Reddy Info: In this publication, scientists from Colorado State University and the National Center for Genome Resources … more »
Posted in Citation
Tagged BluePippin, isoseq, PacBio, RNAseq, transcriptome
Comments Off on A survey of the sorghum transcriptome using single-molecule long reads
PELE-seq Increases SNP Calling Accuracy by Reducing Sequencing Errors
Scientists at the University of Oregon have published a new method to detect PCR and sequencing errors that should help other researchers track rare SNPs with greater accuracy. PELE-seq, which gets our vote for best new protocol name, can be … more »
Posted in Blog
Tagged ddRADseq, genotyping, HiSeq, Illumina, Methods, Pippin Prep
Comments Off on PELE-seq Increases SNP Calling Accuracy by Reducing Sequencing Errors
High-specificity detection of rare alleles with Paired-End Low Error Sequencing (PELE-Seq)
June 2016 Authors: Jessica L. Preston, Ariel E. Royall, Melissa A. Randel, Kristin L. Sikkink, Patrick C. Phillips, and Eric A. Johnson Info: Scientists at the University of Oregon developed a new protocol to reduce sequencing and PCR errors, resulting … more »
Posted in Citation
Comments Off on High-specificity detection of rare alleles with Paired-End Low Error Sequencing (PELE-Seq)
Low-Coverage Sequencing No Challenge for Structural Variant Detection
Is it really possible to detect somatic structural variants accurately from a single sequencing read? A new protocol from scientists at the Albert Einstein College of Medicine in New York and Voronezh State University in Russia was designed to do … more »
Posted in Blog
Tagged Ion Torrent, PippinHT
Comments Off on Low-Coverage Sequencing No Challenge for Structural Variant Detection
Quantitative detection of low-abundance somatic structural variants in normal cells by high-throughput sequencing
June 2016 Authors: Wilber Quispe-Tintaya, Tatyana Gorbacheva, Moonsook Lee, Sergei Makhortov, Vasily N Popov, Jan Vijg & Alexander Y Maslov Info: Scientists report Structural Variant Search, a new method for detecting rare somatic structural variants even with low-coverage sequencing data. … more »
Sage Sizing Platforms Recommended for 10x Genomics Libraries
We’re pleased to report that 10x Genomics has released a new sample prep protocol for its Chromium platform that includes the BluePippin and PippinHT size selection platforms from Sage Science. 10x Genomics has gotten a lot of attention in recent … more »
Posted in Blog
Tagged 10X, BluePippin, PippinHT
Comments Off on Sage Sizing Platforms Recommended for 10x Genomics Libraries
Double Pippin for Optimized RAD-seq
Scientists in China and the UK recently published an open-access optimized protocol for RAD-seq in the Theoretical and Applied Genetics journal. The method is targeted at large studies of plants and enables users to specify sequence coverage parameters. From lead … more »
Podcast: Kari Stefansson on 20 Years of Human Genomics
Mendelspod has turned out another terrific podcast, this one with Kari Stefansson, and we’re proud to have sponsored the thought-provoking discussion. As most people in the field know, Stefansson earned his fame as founder of DeCode Genetics, which has spent … more »
Scientists Use Multiple Technologies to Produce High-Quality Chinese Genome Assembly
A newly reported genome assembly of a Chinese individual, generated by scientists in China and the US, used long-read PacBio sequencing, short-read Illumina data, and BioNano Genomics physical maps to achieve remarkably high accuracy and contiguity. Along the way, the … more »
Posted in Blog
Tagged BluePippin, PacBio
Comments Off on Scientists Use Multiple Technologies to Produce High-Quality Chinese Genome Assembly