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Monthly Archives: March 2016
New hyRAD Method Expands Use of RAD-seq to Degraded Samples
Since RAD-seq was first developed, we’ve seen a number of new versions and approaches from an enthusiastic scientific community. The latest was recently published in PLoS One and demonstrates a RAD-based method suitable for analyzing degraded DNA, an essential step … more »
Posted in Blog
Tagged genotyping, Illumina, Methods, MiSeq, Pippin Prep, RAD-seq
Comments Off on New hyRAD Method Expands Use of RAD-seq to Degraded Samples
Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens
March 2016 Authors: Tomasz Suchan, Camille Pitteloud, Nadezhda S. Gerasimova, Anna Kostikova, Sarah Schmid, Nils Arrigo, Mila Pajkovic, Michał Ronikier, and Nadir Alvarez Info: Scientists present a new protocol based on the RAD-seq concept that works more effectively for samples … more »
Posted in Citation
Tagged Pippin Prep, RAD-seq
Comments Off on Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens
Identification of Human Islet Amyloid Polypeptide as a BACE2 Substrate
February 2016 Authors: Ingrid C. Rulifson, Ping Cao, Li Miao, David Kopecky, Linda Huang, Ryan D. White, Kim Samayoa, Jonitha Gardner, Xiaosu Wu, Kui Chen, Trace Tsuruda, Oliver Homann, Helene Baribault, Harvey Yamane, Tim Carlson, Jed Wiltzius, Yang Li Info: … more »
Posted in Citation
Tagged HiSeq, Illumina, Pippin Prep, RNA sequencing
Comments Off on Identification of Human Islet Amyloid Polypeptide as a BACE2 Substrate
Pitfalls of haplotype phasing from amplicon-based long-read sequencing
February 2016 Authors: Thomas W. Laver, Richard C. Caswell, Karen A. Moore, Jeremie Poschmann, Matthew B. Johnson, Martina M. Owens, Sian Ellard, Konrad H. Paszkiewicz & Michael N. Weedon Info: University of Exeter researchers used long-range PCR and long-read sequencing … more »
Posted in Citation
Tagged BluePippin
Comments Off on Pitfalls of haplotype phasing from amplicon-based long-read sequencing
Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis
February 2016 Authors: Andrea D. Tyler, Sara Christianson, Natalie C. Knox, Philip Mabon, Joyce Wolfe, Gary Van Domselaar, Morag R. Graham, and Meenu K. Sharma Info: In this PLoS One publication, scientists in Manitoba compare the quality of libraries constructed … more »
Posted in Citation
Tagged BluePippin, DNA Sequencing, Illumina, MiSeq
Comments Off on Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis
The Many Dimensions of Diet Breadth: Phytochemical, Genetic, Behavioral, and Physiological Perspectives on the Interaction between a Native Herbivore and an Exotic Host
February 2016 Authors: Joshua G. Harrison, Zachariah Gompert, James A. Fordyce, C. Alex Buerkle, Rachel Grinstead, Joshua P. Jahner, Scott Mikel, Christopher C. Nice, Aldrin Santamaria, and Matthew L. Forister Info: In this study, scientists analyzed butterflies colonizing several different … more »
Posted in Citation
Tagged BluePippin
Comments Off on The Many Dimensions of Diet Breadth: Phytochemical, Genetic, Behavioral, and Physiological Perspectives on the Interaction between a Native Herbivore and an Exotic Host
Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures
January 2016 Authors: Lowiese M. B. Desmarets, Ben L. Vermeulen, Sebastiaan Theuns, Nádia Conceição-Neto, Mark Zeller, Inge D. M. Roukaerts, Delphine D. Acar, Dominique A. J. Olyslaegers, Marc Van Ranst, Jelle Matthijnssens, and Hans J. Nauwyncka Info: Scientists in Belgium … more »
Posted in Citation
Tagged BluePippin
Comments Off on Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures
Transcriptome profiling of Kentucky bluegrass (Poa pratensis L.) accessions in response to salt stress
January 2016 Authors: B. Shaun Bushman, Keenan L. Amundsen, Scott E. Warnke, Joseph G. Robins, and Paul G. Johnson Info: Scientists compared the transcriptomes of two strains of Kentucky bluegrass, one sensitive to salt and the other tolerant even to … more »
Posted in Citation
Tagged BluePippin
Comments Off on Transcriptome profiling of Kentucky bluegrass (Poa pratensis L.) accessions in response to salt stress