Tag Archives: RNA-seq

Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae

April 2013 Authors: Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen Info: The Pippin Prep was used to size-select Illumina RNA-seq libraries. Citation: Int. J. Mol. Sci. 2013, 14, 9080-9098 http://dx.doi.org/10.3390/ijms14059080

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At MIT, Pippin Enables Splice Variant Detection and MicroRNA Analysis

At the BioMicro Center at MIT, Director Stuart Levine, PhD, recently introduced the Pippin Prep from Sage Science to enable key applications — including splice variant analysis with RNA-seq and microRNA analysis — that were not possible on other platforms. … more »

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Publication: Spike-In Standards for Managing Systematic Sequencing Errors

Here’s a paper worth checking out: “Synthetic Spike-in Standards Improve Run-Specific Systematic Error Analysis for DNA and RNA Sequencing” from lead author Justin Zook at the National Institute of Standards and Technology. Published in PLoS One last month, the paper … more »

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Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue

August 2012 Authors: John D. Morlan, Kunbin Qu, Dominick V. Sinicropi Info: After selective depletion of rRNA by RNAse H digestion (following hybridization to rDNA probes), undigested RNA was prepared for NGS sequencing using Illumina TruSeq or Epicentre Script-seq kits. … more »

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Synthetic Spike-in Standards Improve Run-Specific Systematic Error Analysis for DNA and RNA Sequencing

July 2012 Authors: Justin M. Zook, Daniel Samarov, Jennifer McDaniel, Shurjo K. Sen, Marc Salit Info: New approach to discovery of systematic sequencing errors by use of spike in RNA or DNA control DNAs. Pippin Prep used for RNA-seq library … more »

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