Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue

August 2012

John D. Morlan, Kunbin Qu, Dominick V. Sinicropi

After selective depletion of rRNA by RNAse H digestion (following hybridization to rDNA probes), undigested RNA was prepared for NGS sequencing using Illumina TruSeq or Epicentre Script-seq kits. For size-selection steps in the Illumina protocols, Pippin Prep was used for some samples.

PLoS ONE 7(8): e42882. doi:10.1371/journal.pone.0042882

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