John D. Morlan, Kunbin Qu, Dominick V. Sinicropi
After selective depletion of rRNA by RNAse H digestion (following hybridization to rDNA probes), undigested RNA was prepared for NGS sequencing using Illumina TruSeq or Epicentre Script-seq kits. For size-selection steps in the Illumina protocols, Pippin Prep was used for some samples.
PLoS ONE 7(8): e42882. doi:10.1371/journal.pone.0042882