-
Recent Posts
- Leveraging the power of long reads for targeted sequencing
- Resolving the 22q11.2 deletion using CTLR-Seq reveals chromosomal rearrangement mechanisms and individual variance in breakpoints
- CTLR-Seq Protocol
- Processing frozen archival human DNA samples for large-scale SQK-LSK114 Oxford Nanopore long-read DNA sequencing SOP v1
- Identifying the best PCR enzyme for library amplification in NGS
Tags
10x Genomics ABRF agbt Amplicon ASHG ASMS BluePippin ChIP-Seq ddRADseq DNA Sequencing Featured genotyping High Pass HiSeq HLA HLS HLS-CATCH Illumina ILMN Ion Torrent isoseq Mate-pair Sequencing Methods microRNA miRNA miRNA isolation MiSeq ONT oxford nanopore PacBio PAG Paired-end Sequencing PippinHT Pippin Prep Pippin System podcast Protein Mass Spec Protein Size Fractionation proteomics RAD-seq RNA-seq RNA sequencing SageELF SageHLS Tips and ApplicationsRecent Comments
- Alex on And Now, a Word from Our Customer Service Department
- Donna on And Now, a Word from Our Customer Service Department
- Tony Gill on And Now, a Word from Our Customer Service Department
- Alex on And Now, a Word from Our Customer Service Department
- Andrea on And Now, a Word from Our Customer Service Department
Archives
- December 2024
- July 2024
- June 2024
- April 2024
- February 2024
- January 2024
- December 2023
- August 2023
- June 2023
- March 2023
- February 2023
- January 2023
- November 2022
- September 2022
- April 2022
- January 2022
- September 2021
- July 2021
- June 2021
- May 2021
- February 2021
- January 2021
- November 2020
- October 2020
- July 2020
- May 2020
- April 2020
- March 2020
- February 2020
- January 2020
- November 2019
- August 2019
- July 2019
- June 2019
- May 2019
- April 2019
- March 2019
- February 2019
- January 2019
- December 2018
- October 2018
- September 2018
- May 2018
- April 2018
- March 2018
- February 2018
- January 2018
- December 2017
- November 2017
- October 2017
- September 2017
- August 2017
- July 2017
- June 2017
- May 2017
- April 2017
- March 2017
- February 2017
- January 2017
- December 2016
- November 2016
- October 2016
- September 2016
- August 2016
- July 2016
- June 2016
- May 2016
- April 2016
- March 2016
- February 2016
- January 2016
- December 2015
- November 2015
- October 2015
- September 2015
- August 2015
- July 2015
- June 2015
- May 2015
- April 2015
- March 2015
- February 2015
- January 2015
- December 2014
- November 2014
- October 2014
- September 2014
- August 2014
- July 2014
- June 2014
- May 2014
- April 2014
- March 2014
- February 2014
- January 2014
- December 2013
- November 2013
- October 2013
- September 2013
- August 2013
- July 2013
- June 2013
- May 2013
- April 2013
- March 2013
- February 2013
- January 2013
- December 2012
- November 2012
- October 2012
- September 2012
- August 2012
- July 2012
- June 2012
- May 2012
- April 2012
- March 2012
- February 2012
- December 2011
- October 2011
- September 2011
- June 2011
- February 2011
- November 2010
- October 2010
- August 2010
Tag Archives: Pippin System
Improved Protocols for Illumina Sequencing
October 2013 Authors: Iraad F. Bronner, Michael A. Quail, Daniel J. Turner, and Harold Swerdlow Info: A comprehensive overview of the state-of-the state of Illumina sequencing, from the Wellcome Trust Sanger Institute and Oxford Nanopore Technologies. The value of narrow … more »
Posted in Citation
Tagged DNA Sequencing, Featured, Illumina, Pippin Prep, Pippin System
Comments Off on Improved Protocols for Illumina Sequencing
In Skin Microbiome Study, Scientists Find Host Genotype Influences Bacterial Populations
A paper that came out in the Journal of Innate Immunity this summer reports that a person’s genotype affects which microbes will colonize his or her skin, which in turn may alter that person’s defense mechanisms against pathogenic organisms. “Skin … more »
Posted in Blog
Tagged Amplicon, Illumina, Pippin Prep, Pippin System
Comments Off on In Skin Microbiome Study, Scientists Find Host Genotype Influences Bacterial Populations
See You at BioConference Live: Genetics and Genomics
This week brings a new experience for us: Sage is participating in a virtual conference! For those of you not yet familiar with it, BioConference Live runs several meetings each year, all 100 percent online. Attendees can tune in for … more »
Posted in Blog
Tagged Pippin System
Comments Off on See You at BioConference Live: Genetics and Genomics
De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana
August 2013 Authors: Stephen M Gross, Jeffrey A Martin, June Simpson, María Jazmín Abraham-Juarez, Zhong Wang and Axel Visel Info: Agave is an interesting plant for bioenergy study. This paper provides transcriptome data of two strains. The Pippin Prep was … more »
Posted in Citation
Tagged Illumina, Pippin Prep, Pippin System, RNA-seq
Comments Off on De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana
Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses
Authors: Sanne P. Smeekens, c Curtis Huttenhower, Anca Riza, Frank L. van de Veerdonk, Patrick L.J.M. Zeeuwen, Joost Schalkwijk, Jos W.M. van der Meer, Ramnik J. Xavier, Mihai G. Netea, Dirk Gevers Info: Genomic DNA was subjected to 16S PCR … more »
Posted in Citation
Tagged Amplicon, Illumina, Pippin Prep, Pippin System
Comments Off on Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses
A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology
June 2013 Authors: A. Murat Eren, Joseph H. Vineis., Hilary G. Morrison, Mitchell L. Sogin Info: The authors present a method of library preparation for overlapping paired-end sequencing on the Illumina platform, and improved analysis software, to reduce the error … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep, Pippin System
Comments Off on A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology
Deciphering the Growth Behaviour of Mycobacterium africanum
May 2013 Authors: Florian Gehre, Jacob Otu, Kathryn DeRiemer, Paola Florez de Sessions, Martin L. Hibberd, Wim Mulders, Tumani Corrah, Bouke C. de Jong, Martin Antonio Info: The Pippin Prep was used to size select libraries prepared with the New … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep, Pippin System
Comments Off on Deciphering the Growth Behaviour of Mycobacterium africanum
FOXO1 regulates expression of a microRNA cluster on X chromosome
May 2013 Authors: Ruchi Singhal, Jonathan E. Bard, Norma J. Nowak, Michael J.Buck, and Eugene S. Kandel Info: The Pippin Prep was used in size selection with the Illumina TruSeq Small RNA sequencing protocol. Citation: AGING 5(5): 347-356 http://www.impactaging.com/papers/v5/n5/full/100558.html
Posted in Citation
Tagged Illumina, Pippin Prep, Pippin System, RNA-seq
Comments Off on FOXO1 regulates expression of a microRNA cluster on X chromosome
Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
April 2013 Authors: Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson Info: The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification … more »
Posted in Citation
Tagged Amplicon, DNA Sequencing, Featured, Pippin Prep, Pippin System
Comments Off on Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies
Before a new technology is deployed at The Genome Analysis Centre in Norwich, UK, it must run the gauntlet of Matthew Clark’s lab. Clark is the sequencing technology development leader at TGAC, where part of his role involves testing out … more »
Posted in Blog
Tagged BluePippin, DNA Sequencing, Illumina, Mate-pair Sequencing, PacBio, Pippin System
Comments Off on At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies