Tag Archives: Pippin Prep

A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling

January 2016 Authors: Rosalinda D’Amore, Umer Zeeshan Ijaz, Melanie Schirmer, John G. Kenny, Richard Gregory, Alistair C. Darby, Migun Shakya, Mircea Podar, Christopher Quince and Neil Hall Info: Scientists used synthetic metagenomic communities to evaluate the performance of different sequencing … more »

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Harnessing the power of RADseq for ecological and evolutionary genomics

January 2016 Authors: Kimberly R. Andrews, Jeffrey M. Good, Michael R. Miller, Gordon Luikart and Paul A. Hohenlohe Info: In this protocol review, scientists examine several approaches to restriction site-associated DNA sequencing and present their findings on how to choose … more »

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For MicroRNA Discovery, Pippin Prep Delivers Highest Yield and Specificity

In a highly accessed paper in BMC Medical Genomics, scientists from McGill University and EMBL tested several steps to find the most robust pipeline for discovering small non-coding RNAs (sncRNAs) that might be useful as biomarkers. As part of this … more »

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Next generation sequencing to identify novel genetic variants causative of autosomal dominant familial hypercholesterolemia associated with increased risk of coronary heart disease

July 2015 Authors: Faisal A. Al-Allaf, Mohammad Athar, Zainularifeen Abduljaleel, Mohiuddin M. Taher, Wajahatullah Khan, Faisal A. Ba-hammam, Hala Abalkhail, Abdullah Alashwal Info: Scientists from Saudi Arabia published a paper in Gene demonstrating the use of targeted sequencing to detect … more »

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Isolation via next-generation sequencing of microsatellites from the Tasmanian macroalgae Lessonia corrugata (Lessoniaceae)

July 2015 Authors: Halley M. S. Durrant, Christopher P. Burridge, and Michael G. Gardner Info: Scientists from Australia developed microsatellite markers for this macroalgae, an important component of the local marine ecosystem, that will allow for future population genetic studies … more »

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A genomic selection component analysis characterizes migration-selection balance

July 2015 Authors: Patrick J. Monnahan, Jack Colicchio, and John K. Kelly Info: In the journal Evolution, scientists from the University of Kansas report the population study of a plant species in novel habitat, which allowed them to carefully monitor … more »

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Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath

July 2015 Authors: Sandeep K. Kasoji, Samantha G. Pattenden, Ewa P. Malc, Chatura N. Jayakody, James K. Tsuruta, Piotr A. Mieczkowski, William P. Janzen, Paul A. Dayton Info: This paper from researchers at the University of North Carolina describes the … more »

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KRAS-dependent sorting of miRNA to exosomes

July 2015 Authors: Diana J Cha, Jeffrey L Franklin, Yongchao Dou, Qi Liu, James N Higginbotham, Michelle Demory Beckler, Alissa M Weaver, Kasey Vickers, Nirpesh Prasad, Shawn Levy, Bing Zhang, Robert J Coffey, and James G Patton Info: Scientists from … more »

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Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing

July 2015 Authors: Juan Pablo Lopez, Alpha Diallo, Cristiana Cruceanu, Laura M. Fiori, Sylvie Laboissiere, Isabelle Guillet, Joelle Fontaine, Jiannis Ragoussis, Vladimir Benes, Gustavo Turecki, and Carl Ernst Info: Scientists from McGill University and the European Molecular Biology Laboratory evaluated … more »

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Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo

July 2015 Authors: Yi-Zhou Jiang, Elisabetta Manduchi, Christian J. Stoeckert, and Peter F. Davies Info: In this paper, scientists from the University of Pennsylvania used complete methylated DNA immunoprecipitation sequencing (MeDIP-seq) to study methylation differences in endothelial cells to find … more »

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