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Tag Archives: Pippin Prep
In Skin Microbiome Study, Scientists Find Host Genotype Influences Bacterial Populations
A paper that came out in the Journal of Innate Immunity this summer reports that a person’s genotype affects which microbes will colonize his or her skin, which in turn may alter that person’s defense mechanisms against pathogenic organisms. “Skin … more »
Posted in Blog
Tagged Amplicon, Illumina, Pippin Prep, Pippin System
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De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana
August 2013 Authors: Stephen M Gross, Jeffrey A Martin, June Simpson, María Jazmín Abraham-Juarez, Zhong Wang and Axel Visel Info: Agave is an interesting plant for bioenergy study. This paper provides transcriptome data of two strains. The Pippin Prep was … more »
Posted in Citation
Tagged Illumina, Pippin Prep, Pippin System, RNA-seq
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Comparative genomics of Campylobacter concisus isolates reveals genetic diversity and provides insights into disease association
August 2013 Authors: Nandan P Deshpande, Nadeem O Kaakoush, Marc R Wilkins and Hazel M Mitchell Info: Collaborators at the University of New South Wales sequence several strains of Campylobacter concisus from healthy and diseased (Crohn’s disease and gastroenteritis) individuals … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep
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A quantitative reference transcriptome for Nematostella vectensis early embryonic development: a pipeline for de novo assembly in emerging model systems
June 2013 Authors: Sarah Tulin, Derek Aguiar, Sorin Istrail, and Joel Smith Info: Scientists from the Marine Biological Laboratory and Brown University developed a new pipeline for de novo transcriptome assembly that can be used with organisms lacking a well-annotated … more »
Posted in Citation
Tagged Illumina, Pippin Prep, RNA sequencing, RNA-seq, ScriptSeq
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Phylogeographic Origin of Helicobacter pylori Determines Host-Adaptive Responses upon Coculture with Gastric Epithelial Cells
July 2013 Authors: Alexander Sheh, Rupesh Chaturvedi, D. Scott Merrell, Pelayo Correa, Keith T. Wilson, James G. Fox Info: A study of gene expression comparing several strains of H. pylori. The BluePippin was used to size-select fragments to construct paired-end … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep
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The new sequencer on the block: comparison of Life Technology’s Proton sequencer to an Illumina HiSeq for whole-exome sequencing
June 2013 Authors: Joseph F. Boland, Charles C. Chung, David Roberson, Jason Mitchell, Xijun Zhang, Kate M. Im, Ji He, Stephen J. Chanock, Meredith Yeager, Michael Dean Info: An exome-sequencing study from the NIH comparing Illumina/Nimblegen to Ion Torrent/TargetSeq. Pippin … more »
Posted in Citation
Tagged DNA Sequencing, Ion Torrent, Pippin Prep
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Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses
Authors: Sanne P. Smeekens, c Curtis Huttenhower, Anca Riza, Frank L. van de Veerdonk, Patrick L.J.M. Zeeuwen, Joost Schalkwijk, Jos W.M. van der Meer, Ramnik J. Xavier, Mihai G. Netea, Dirk Gevers Info: Genomic DNA was subjected to 16S PCR … more »
Posted in Citation
Tagged Amplicon, Illumina, Pippin Prep, Pippin System
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A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology
June 2013 Authors: A. Murat Eren, Joseph H. Vineis., Hilary G. Morrison, Mitchell L. Sogin Info: The authors present a method of library preparation for overlapping paired-end sequencing on the Illumina platform, and improved analysis software, to reduce the error … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep, Pippin System
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New Prostate Cancer Research Indicates Rapid Accumulation of DNA Mutations
Researchers studying the behavior of prostate cancer have shed light on how the cancer develops mutations so rapidly. In contrast to other cancers that seem to progress by adding mutations one at a time, the prostate cancer samples that were … more »
Posted in Blog
Tagged DNA Sequencing, Pippin Prep
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Deciphering the Growth Behaviour of Mycobacterium africanum
May 2013 Authors: Florian Gehre, Jacob Otu, Kathryn DeRiemer, Paola Florez de Sessions, Martin L. Hibberd, Wim Mulders, Tumani Corrah, Bouke C. de Jong, Martin Antonio Info: The Pippin Prep was used to size select libraries prepared with the New … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep, Pippin System
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