A quantitative reference transcriptome for Nematostella vectensis early embryonic development: a pipeline for de novo assembly in emerging model systems

June 2013

Sarah Tulin, Derek Aguiar, Sorin Istrail, and Joel Smith

Scientists from the Marine Biological Laboratory and Brown University developed a new pipeline for de novo transcriptome assembly that can be used with organisms lacking a well-annotated reference genome. The paper describes library preparation and computational analysis processes. Pippin Prep was used for size selection in a standard Illumina workflow and also in conjunction with an Epicentre ScriptSeq™ kit.

EvoDevo 2013 4:16


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