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Tag Archives: Paired-end Sequencing
A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology
June 2013 Authors: A. Murat Eren, Joseph H. Vineis., Hilary G. Morrison, Mitchell L. Sogin Info: The authors present a method of library preparation for overlapping paired-end sequencing on the Illumina platform, and improved analysis software, to reduce the error … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep, Pippin System
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Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene
January 2013 Authors: Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins Info: The authors designed a general method to study the arrangement of foreign DNA and host … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep
Comments Off on Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene
Bactobolin Resistance is Conferred by Mutations in the L2 Ribosomal Protein
December 2012 Authors: Josephine Chandler, Thao T. Truong, Patricia M. Silva, Mohammad R. Seyedsayamdost, Galvin Carr, Matthew Radey, Michael A. Jacobs, Elizabeth H. Sims, Jon Clardy, E. Peter Greenberg Info: Authors were searching for cause of resistance to antibiotic Bactobolin. … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep
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The Use of NexGen Sequencing and Junction Sequence Analysis Bioinformatics to Achieve Molecular Characterization of Crops Improved Through Modern Biotechnology
October 2012 Authors: David Kovalic, Carl Garnaat, Liang Guo, Yongpan Yan, Jeanna Groat, Andre Silvanovich, Lyle Ralston, Mingya Huang, Qing Tian, Allen Christian, Nordine Cheikh, Jerry Hjelle, Stephen Padgette and Gary Bannon Info: Materials and Methods: Illumina TruSeq paired-end library … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep
Comments Off on The Use of NexGen Sequencing and Junction Sequence Analysis Bioinformatics to Achieve Molecular Characterization of Crops Improved Through Modern Biotechnology
Mapping the Hallmarks of Lung Adenocarcinoma with Massively Parallel Sequencing
September 2012 Authors: Marcin Imielinski et al. Info: A landmark study of lung cancer genomes with 183 tumor/normal genomes compared. Sequencing was carried out mainly by the Broad Illumina Platform (8 Pippins in production). The Pippin Prep is cited in … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep
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For NRC Team, Pippin Platform Leads to Higher-Quality Assemblies
At the DNA Technologies Laboratory at the National Research Council of Canada, scientists are using the Pippin size selection platform to improve the quality of their genome assemblies. Andrew Sharpe, Research Officer and Group Leader of the Saskatoon-based laboratory, got … more »
Posted in Blog
Tagged BluePippin, DNA Sequencing, Illumina, Mate-pair Sequencing, Paired-end Sequencing, Pippin Prep, Pippin System
Comments Off on For NRC Team, Pippin Platform Leads to Higher-Quality Assemblies
Low Incidence of DNA Sequence Variation in Human Induced Pluripotent Stem Cells Generated by Nonintegrating Plasmid Expression
March 2012 Authors: Linzhao Cheng, Nancy F. Hansen, Ling Zhao, Yutao Du, Chunlin Zou, Frank X. Donovan, Bin-Kuan Chou, Guangyu Zhou, Shijie Li, Sarah N. Dowey, Zhaohui Ye, NISC Comparative Sequencing Program, Settara C. Chandrasekharappa, Huanming Yang, James C. Mullikin, … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep, Pippin System
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Mutation discovery in mice by whole exome sequencing
December 2011 Authors: Heather Fairfield, Griffith J Gilbert, Mary Barter, Rebecca R Corrigan, Michelle Curtain, Yueming Ding, Mark D’Ascenzo, Daniel J Gerhardt, Chao He, Wenhui Huang, Todd Richmond, Lucy Rowe, Frank J Probst, David E Bergstrom, Stephen A Murray, Carol … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep, Pippin System
Comments Off on Mutation discovery in mice by whole exome sequencing
Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn
September 2011 Authors: Laura G. Reinholdt, Yueming Ding, Griffith T. Gilbert, Anne Czechanski, Jeffrey P. Solzak, Randall J. Roper, Mark T. Johnson, Leah Rae Donahue, Cathleen Lutz, Muriel T. Davisson Info: Illumina PE libraries were constructed and size selected at … more »
Posted in Citation
Tagged DNA Sequencing, Paired-end Sequencing, Pippin Prep, Pippin System
Comments Off on Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn