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Tag Archives: Illumina
Multiplex target capture with double-stranded DNA probes
May 2013 Authors: Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe Info: The authors describe a new method for targeted NGS using double-stranded probes rather than single-stranded ones, improving sensitivity for variant detection, and at … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep
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FOXO1 regulates expression of a microRNA cluster on X chromosome
May 2013 Authors: Ruchi Singhal, Jonathan E. Bard, Norma J. Nowak, Michael J.Buck, and Eugene S. Kandel Info: The Pippin Prep was used in size selection with the Illumina TruSeq Small RNA sequencing protocol. Citation: AGING 5(5): 347-356 http://www.impactaging.com/papers/v5/n5/full/100558.html
Posted in Citation
Tagged Illumina, Pippin Prep, Pippin System, RNA-seq
Comments Off on FOXO1 regulates expression of a microRNA cluster on X chromosome
At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies
Before a new technology is deployed at The Genome Analysis Centre in Norwich, UK, it must run the gauntlet of Matthew Clark’s lab. Clark is the sequencing technology development leader at TGAC, where part of his role involves testing out … more »
Posted in Blog
Tagged BluePippin, DNA Sequencing, Illumina, Mate-pair Sequencing, PacBio, Pippin System
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Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae
April 2013 Authors: Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen Info: The Pippin Prep was used to size-select Illumina RNA-seq libraries. Citation: Int. J. Mol. Sci. 2013, 14, 9080-9098 http://dx.doi.org/10.3390/ijms14059080
Posted in Citation
Tagged Illumina, Pippin Prep, Pippin System, RNA-seq
Comments Off on Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae
Pippin Shines in Size Selection Comparison from Sanger
A paper that came out December in the journal Electrophoresis compares Pippin Prep size selection with Caliper’s Labchip XT and finds that Pippin gives “very tight size distributions” not seen with other sizing methods. “Evaluation and optimisation of preparative semi-automated … more »
Posted in Blog
Tagged DNA Sequencing, Illumina, Pippin Prep
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Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential
January/February 2013 Authors: Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana Info: Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep
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Pippin Platform Recommended for Nextera Mate Pair Size Selection
Illumina released new sample prep protocol guidelines for generating mate pair libraries with its Nextera kit, and we’re pleased to report that the Pippin platform is the recommended choice for automated size selection. You can check out the Nextera Mate … more »
Posted in Blog
Tagged Illumina, Mate-pair Sequencing, Pippin Prep
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Tag Team: At DFCI, Pippin and Nextera Make Better Libraries
Over at the Dana-Farber Cancer Institute, scientists have been doing some really interesting work pairing the Pippin Prep with Illumina’s Nextera kit for library preparation. Zach Herbert, associate director of the Molecular Biology Core Facilites at DFCI, says that he … more »
Posted in Blog
Tagged Amplicon, DNA Sequencing, Illumina, Pippin Prep
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Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene
January 2013 Authors: Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins Info: The authors designed a general method to study the arrangement of foreign DNA and host … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep
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Bactobolin Resistance is Conferred by Mutations in the L2 Ribosomal Protein
December 2012 Authors: Josephine Chandler, Thao T. Truong, Patricia M. Silva, Mohammad R. Seyedsayamdost, Galvin Carr, Matthew Radey, Michael A. Jacobs, Elizabeth H. Sims, Jon Clardy, E. Peter Greenberg Info: Authors were searching for cause of resistance to antibiotic Bactobolin. … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep
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