Tag Archives: Illumina

Multiplex target capture with double-stranded DNA probes

Posted on May 23, 2013 by Alex

May 2013 Authors: Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe Info: The authors describe a new method for targeted NGS using double-stranded probes rather than single-stranded ones, improving sensitivity for variant detection, and at … more »

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FOXO1 regulates expression of a microRNA cluster on X chromosome

Posted on May 2, 2013 by Alex

May 2013 Authors: Ruchi Singhal, Jonathan E. Bard, Norma J. Nowak, Michael J.Buck, and Eugene S. Kandel Info: The Pippin Prep was used in size selection with the Illumina TruSeq Small RNA sequencing protocol. Citation: AGING 5(5): 347-356 http://www.impactaging.com/papers/v5/n5/full/100558.html

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At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies

Posted on April 16, 2013 by Alex

Before a new technology is deployed at The Genome Analysis Centre in Norwich, UK, it must run the gauntlet of Matthew Clark’s lab. Clark is the sequencing technology development leader at TGAC, where part of his role involves testing out … more »

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Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae

Posted on April 10, 2013 by Alex

April 2013 Authors: Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen Info: The Pippin Prep was used to size-select Illumina RNA-seq libraries. Citation: Int. J. Mol. Sci. 2013, 14, 9080-9098 http://dx.doi.org/10.3390/ijms14059080

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Pippin Shines in Size Selection Comparison from Sanger

Posted on March 13, 2013 by Alex

A paper that came out December in the journal Electrophoresis compares Pippin Prep size selection with Caliper’s Labchip XT and finds that Pippin gives “very tight size distributions” not seen with other sizing methods. “Evaluation and optimisation of preparative semi-automated … more »

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Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential

Posted on February 1, 2013 by Alex

January/February 2013 Authors: Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana Info: Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome … more »

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Pippin Platform Recommended for Nextera Mate Pair Size Selection

Posted on January 24, 2013 by Alex

Illumina released new sample prep protocol guidelines for generating mate pair libraries with its Nextera kit, and we’re pleased to report that the Pippin platform is the recommended choice for automated size selection. You can check out the Nextera Mate … more »

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Tag Team: At DFCI, Pippin and Nextera Make Better Libraries

Posted on January 18, 2013 by Alex

Over at the Dana-Farber Cancer Institute, scientists have been doing some really interesting work pairing the Pippin Prep with Illumina’s Nextera kit for library preparation. Zach Herbert, associate director of the Molecular Biology Core Facilites at DFCI, says that he … more »

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Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene

Posted on January 11, 2013 by Alex

January 2013 Authors: Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins Info: The authors designed a general method to study the arrangement of foreign DNA and host … more »

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Bactobolin Resistance is Conferred by Mutations in the L2 Ribosomal Protein

Posted on December 1, 2012 by Alex

December 2012 Authors: Josephine Chandler, Thao T. Truong, Patricia M. Silva, Mohammad R. Seyedsayamdost, Galvin Carr, Matthew Radey, Michael A. Jacobs, Elizabeth H. Sims, Jon Clardy, E. Peter Greenberg Info: Authors were searching for cause of resistance to antibiotic Bactobolin. … more »

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