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Tag Archives: DNA Sequencing
H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis
March/April 2013 Authors: Kyle C. Costa, Thomas J. Lie, Michael A. Jacobs, John A. Leigh Info: The authors constructed deletions in several key metabolism genes to study the mechanism of H2 independent growth in this hydrogenotropic strain. Several mutants were … more »
Posted in Citation
Tagged DNA Sequencing, Pippin Prep
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At Iowa Core Lab, Pippin Sizing Is Essential for MicroRNA Studies
Kevin Knudtson’s DNA Facility at the University of Iowa acquired a Pippin Prep almost a year ago, and in the time since, the automated size selection tool has become an integral part of the core lab’s microRNA pipeline. “Pippin is … more »
Posted in Blog
Tagged ChIP-Seq, DNA Sequencing, miRNA isolation, Pippin Prep
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Pippin Shines in Size Selection Comparison from Sanger
A paper that came out December in the journal Electrophoresis compares Pippin Prep size selection with Caliper’s Labchip XT and finds that Pippin gives “very tight size distributions” not seen with other sizing methods. “Evaluation and optimisation of preparative semi-automated … more »
Posted in Blog
Tagged DNA Sequencing, Illumina, Pippin Prep
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Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential
January/February 2013 Authors: Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana Info: Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep
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A genome-wide survey of genetic variation in gorillas using reduced representation sequencing
January 2013 Authors: Aylwyn Scally, Bryndis Yngvadottir, Yali Xue, Qasim Ayub, Richard Durbin, Chris Tyler-Smith Info: The study used reduced representation libraries to study diversity among the two currently recognized species of gorilla. The libraries were generated by AluI digestion … more »
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Tagged DNA Sequencing, Pippin Prep
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Poster: Exome Sequencing with Ion Torrent and Pippin Prep
In a poster from Ion Torrent (Life Technologies) for 2012’s ASHG, scientists looked at exome sequencing by studying a familial trio on both the PGM™ and the Proton™ instruments. Size selection for both sequencers was performed on the Pippin Prep … more »
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Tagged DNA Sequencing, Ion Torrent, Pippin Prep
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Tag Team: At DFCI, Pippin and Nextera Make Better Libraries
Over at the Dana-Farber Cancer Institute, scientists have been doing some really interesting work pairing the Pippin Prep with Illumina’s Nextera kit for library preparation. Zach Herbert, associate director of the Molecular Biology Core Facilites at DFCI, says that he … more »
Posted in Blog
Tagged Amplicon, DNA Sequencing, Illumina, Pippin Prep
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DNA for Species Identification: A Study of Rodents
Carl Linnaeus would be proud: A recent paper in PLoS One demonstrates the use of next-gen sequencing with genetic barcodes to accurately identify more than 100 different species from the Rodentia order. In the study, amplicons were run on the … more »
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Tagged DNA Sequencing, Pippin Prep
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Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene
January 2013 Authors: Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins Info: The authors designed a general method to study the arrangement of foreign DNA and host … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep
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Discrete genetic modules are responsible for complex burrow evolution in Peromyscus mice
January 2013 Authors: Jesse N. Weber, Brant K. Peterson & Hopi E. Hoekstra Info: The work seeks to identify genetic influences on a complex behavior in mice, burrow formation. The authors used a modified RADseq procedure to genotype two species … more »
Posted in Citation
Tagged DNA Sequencing, RAD-seq
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