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Tag Archives: DNA Sequencing
Back from Baltimore and the Stellar PacBio UGM
Earlier this week Pacific Biosciences hosted a user group meeting at the University of Maryland in Baltimore, and we were pleased to participate in and co-sponsor the event. As our blog readers know, we recently announced a co-marketing partnership with … more »
Posted in Blog
Tagged BluePippin, DNA Sequencing, PacBio
Comments Off on Back from Baltimore and the Stellar PacBio UGM
A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology
June 2013 Authors: A. Murat Eren, Joseph H. Vineis., Hilary G. Morrison, Mitchell L. Sogin Info: The authors present a method of library preparation for overlapping paired-end sequencing on the Illumina platform, and improved analysis software, to reduce the error … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep, Pippin System
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New Prostate Cancer Research Indicates Rapid Accumulation of DNA Mutations
Researchers studying the behavior of prostate cancer have shed light on how the cancer develops mutations so rapidly. In contrast to other cancers that seem to progress by adding mutations one at a time, the prostate cancer samples that were … more »
Posted in Blog
Tagged DNA Sequencing, Pippin Prep
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Deciphering the Growth Behaviour of Mycobacterium africanum
May 2013 Authors: Florian Gehre, Jacob Otu, Kathryn DeRiemer, Paola Florez de Sessions, Martin L. Hibberd, Wim Mulders, Tumani Corrah, Bouke C. de Jong, Martin Antonio Info: The Pippin Prep was used to size select libraries prepared with the New … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep, Pippin System
Comments Off on Deciphering the Growth Behaviour of Mycobacterium africanum
Multiplex target capture with double-stranded DNA probes
May 2013 Authors: Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe Info: The authors describe a new method for targeted NGS using double-stranded probes rather than single-stranded ones, improving sensitivity for variant detection, and at … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep
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Pippin and PacBio: Better Together
Update: Just announced– Sage Science and Pacific Biosciences have partnered to offer users longer reads than ever. Click here to read the press release. The new SMRTbellTM 20kb sample prep protocol using the BluePippin is posted on the PacBio® SampleNet … more »
Posted in Blog
Tagged agbt, BluePippin, DNA Sequencing, PacBio
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Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
April 2013 Authors: Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson Info: The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification … more »
Posted in Citation
Tagged Amplicon, DNA Sequencing, Featured, Pippin Prep, Pippin System
Comments Off on Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies
Before a new technology is deployed at The Genome Analysis Centre in Norwich, UK, it must run the gauntlet of Matthew Clark’s lab. Clark is the sequencing technology development leader at TGAC, where part of his role involves testing out … more »
Posted in Blog
Tagged BluePippin, DNA Sequencing, Illumina, Mate-pair Sequencing, PacBio, Pippin System
Comments Off on At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies
NHGRI Scientists Find Transgenic DNA Has More Rearrangements than Expected
Amanda DuBose didn’t set out to come up with a new technical solution to characterizing transgenic DNA in animal models. A postdoctoral fellow in Francis Collins’ lab at the National Human Genome Research Institute, DuBose was using mice to study … more »
Posted in Blog
Tagged DNA Sequencing, Pippin Prep
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Selective Inhibition of Tumor Oncogenes by Disruption of Super-Enhancers
April 2013 Authors: Jakob Love, Heather A. Hoke, Charles Y. Lin, Ashley Lau, David A. Orlando, Christopher R. Vakoc, James E. Bradner, Tong Ihn Lee, and Richard A. Young Info: The Pippin Prep was used to size-select ChIP-seq libraries. Citation: … more »
Posted in Citation
Tagged ChIP-Seq, DNA Sequencing
Comments Off on Selective Inhibition of Tumor Oncogenes by Disruption of Super-Enhancers