Search Results for: microrna

Pippin Enriches for MicroRNAs in Zebrafish Transcriptome Study

Posted on May 15, 2014 by Alex

Scientists from the Mayo Clinic, Institute for Systems Biology, and University of Illinois recently published a study investigating the effects of vitamin D on microRNA regulation of gene expression in zebrafish. The team used our Pippin Prep together with the … more »

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MicroRNA

Posted on January 24, 2014 by Alex

Pippin instruments can be used for isolation of microRNA libraries from adapter-dimer content and larger cDNAs. The Pippin 3% agarose gel cassette was developed for the preparation of miRNA libraries. Adapter-ligated cDNAs of miRNAs (typically 100-170 base pairs depending on … more »

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Epigenetic Regulation of the DLK1-MEG3 MicroRNA Cluster in Human Type 2 Diabetic Islets

Posted on January 6, 2014 by Alex

January 2014 Authors: Vasumathi Kameswaran, Nuria C. Bramswig, Lindsay B. McKenna, Melinda Penn, Jonathan Schug, Nicholas J. Hand, Ying Chen, Inchan Choi, Anastassios Vourekas, Kyoung-Jae Won, Chengyang Liu, Kumar Vivek, Ali Naji, Joshua R. Friedman, and Klaus H. Kaestner Info: … more »

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FOXO1 regulates expression of a microRNA cluster on X chromosome

Posted on May 2, 2013 by Alex

May 2013 Authors: Ruchi Singhal, Jonathan E. Bard, Norma J. Nowak, Michael J.Buck, and Eugene S. Kandel Info: The Pippin Prep was used in size selection with the Illumina TruSeq Small RNA sequencing protocol. Citation: AGING 5(5): 347-356 http://www.impactaging.com/papers/v5/n5/full/100558.html

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At Iowa Core Lab, Pippin Sizing Is Essential for MicroRNA Studies

Posted on March 21, 2013 by Alex

Kevin Knudtson’s DNA Facility at the University of Iowa acquired a Pippin Prep almost a year ago, and in the time since, the automated size selection tool has become an integral part of the core lab’s microRNA pipeline. “Pippin is … more »

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At MIT, Pippin Enables Splice Variant Detection and MicroRNA Analysis

Posted on February 26, 2013 by Alex

At the BioMicro Center at MIT, Director Stuart Levine, PhD, recently introduced the Pippin Prep from Sage Science to enable key applications — including splice variant analysis with RNA-seq and microRNA analysis — that were not possible on other platforms. … more »

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New BluePippin 2.5% Agarose Cassettes Provide High Size Selection Accuracy Between 100-600bp

Posted on November 5, 2025 by Alex

For Illumina and short-read sequencing applications, BluePippin users typically use either high-resolution 3% agarose gel cassettes and/or slightly lower resolution 2% agarose gel cassettes. The 3% cassettes are great for “threading the needle” when separating microRNA libraries from nearby unincorporated … more »

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Resetting of 24-nt siRNA landscape is initiated before the first zygotic division in rice

Posted on May 19, 2021 by Alex

January 2021 Authors: Chenxin Li, Jonathan I. Gent, Hengping Xu, Hong Fu, Scott D. Russell, and Venkatesan Sundaresan Info: In this preprint, the authors report on a study of siRNA profiling in zygotes from the rice plant. They report that … more »

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Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis

Posted on March 4, 2020 by Alex

February 2020 Authors: Phuong-Uyen C. Dinh, Dipti Paudel, Hayden Brochu, Kristen D. Popowski, M. Cyndell Gracieux, Jhon Cores, Ke Huang, M. Taylor Hensley, Erin Harrell, Adam C. Vandergriff, Arianna K. George, Raina T. Barrio, Shiqi Hu, Tyler A. Allen, Kevin … more »

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Micro RNA’s (miRNA’s) may help explain expression of multiple genes in Alzheimer’s Frontal Cortex

Posted on September 20, 2017 by Alex

September 2017 Authors: James Bennett and Paula Keeney Info: Scientists from Neurodegeneration Therapeutics used RNA-seq to compare microRNAs found in Alzheimer’s brain samples and controls. They identified 55 up-regulated genes and 191 down-regulated genes in the Alzheimer’s samples. PippinHT was … more »

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