Resetting of 24-nt siRNA landscape is initiated before the first zygotic division in rice

January 2021

Chenxin Li, Jonathan I. Gent, Hengping Xu, Hong Fu, Scott D. Russell, and Venkatesan Sundaresan

In this preprint, the authors report on a study of siRNA profiling in zygotes from the rice plant. They report that the zygote has widespread siRNA loci that correlate to the endosperm rather than the egg, and that these loci have abundant CHH methylation. A small fraction of siRNA “siren” loci correlate to the egg and account for 75% of the zygote siRNAs and are not associated with methylation. The results suggest that the resetting of the gametic epigenome toward the canonical vegetative profile initiates before the first embryonic division.

PippinHT is used to isolate the microRNA libraries from NEXTFlex small RNA kits (Perkin Elmer/Bioo Scientific) prior to Illumina sequencing.

Author Affiliations:
Department of Plant Biology, University of California, Davis, CA
Department of Plant Biology, University of Georgia, Athens, GA
Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK
Department of Plant Sciences, University of California, Davis, CA

bioRxiv preprint
DOI: 10.1101/2020.08.31.275958

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