Search Results for: microrna

MicroRNA

Pippin instruments can be used for isolation of microRNA libraries from adapter-dimer content and larger cDNAs. The Pippin 3% agarose gel cassette was developed for the preparation of miRNA libraries. Adapter-ligated cDNAs of miRNAs (typically 100-170 base pairs depending on … more »

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Epigenetic Regulation of the DLK1-MEG3 MicroRNA Cluster in Human Type 2 Diabetic Islets

January 2014 Authors: Vasumathi Kameswaran, Nuria C. Bramswig, Lindsay B. McKenna, Melinda Penn, Jonathan Schug, Nicholas J. Hand, Ying Chen, Inchan Choi, Anastassios Vourekas, Kyoung-Jae Won, Chengyang Liu, Kumar Vivek, Ali Naji, Joshua R. Friedman, and Klaus H. Kaestner Info: … more »

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FOXO1 regulates expression of a microRNA cluster on X chromosome

May 2013 Authors: Ruchi Singhal, Jonathan E. Bard, Norma J. Nowak, Michael J.Buck, and Eugene S. Kandel Info: The Pippin Prep was used in size selection with the Illumina TruSeq Small RNA sequencing protocol. Citation: AGING 5(5): 347-356 http://www.impactaging.com/papers/v5/n5/full/100558.html

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At Iowa Core Lab, Pippin Sizing Is Essential for MicroRNA Studies

Kevin Knudtson’s DNA Facility at the University of Iowa acquired a Pippin Prep almost a year ago, and in the time since, the automated size selection tool has become an integral part of the core lab’s microRNA pipeline. “Pippin is … more »

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At MIT, Pippin Enables Splice Variant Detection and MicroRNA Analysis

At the BioMicro Center at MIT, Director Stuart Levine, PhD, recently introduced the Pippin Prep from Sage Science to enable key applications — including splice variant analysis with RNA-seq and microRNA analysis — that were not possible on other platforms. … more »

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Resetting of 24-nt siRNA landscape is initiated before the first zygotic division in rice

January 2021 Authors: Chenxin Li, Jonathan I. Gent, Hengping Xu, Hong Fu, Scott D. Russell, and Venkatesan Sundaresan Info: In this preprint, the authors report on a study of siRNA profiling in zygotes from the rice plant. They report that … more »

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Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis

February 2020 Authors: Phuong-Uyen C. Dinh, Dipti Paudel, Hayden Brochu, Kristen D. Popowski, M. Cyndell Gracieux, Jhon Cores, Ke Huang, M. Taylor Hensley, Erin Harrell, Adam C. Vandergriff, Arianna K. George, Raina T. Barrio, Shiqi Hu, Tyler A. Allen, Kevin … more »

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Micro RNA’s (miRNA’s) may help explain expression of multiple genes in Alzheimer’s Frontal Cortex

September 2017 Authors: James Bennett and Paula Keeney Info: Scientists from Neurodegeneration Therapeutics used RNA-seq to compare microRNAs found in Alzheimer’s brain samples and controls. They identified 55 up-regulated genes and 191 down-regulated genes in the Alzheimer’s samples. PippinHT was … more »

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Differential regulation of miRNA and mRNA expression in the myocardium of Nrf2 knockout mice

July 2017 Authors: Justin Quiles, Madhusudhanan Narasimhan, Gobinath Shanmugam, Brett Milash, John Hoidal, and Namakkal Rajasekaran Info: Scientists from the University of Alabama at Birmingham and other institutions studied a gene previously associated with oxidative stress, analyzing differential expression in … more »

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The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity

December 2016 Authors: Samer G. Alam, Qiao Zhang, Nripesh Prasad, Yuan Li, Srikar Chamala, et al. Info: Researchers from the HudsonAlpha Institute, the University of Florida, and other institutions studied the role of rigidity in the extracellular matrix, finding that … more »

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