Tricia L. Rubi, L. Lacey Knowles, Ben Dantzer
In this preprint, researchers developed method the study epigenetic markers on historically preserved deer mouse specimens using ddRAD-seq and bisulfite sequencing. The oldest group of specimens was 76 years old.
The Pippin Prep was used to size select ddRAD libraries prepared with an extra bisulfite conversion step. Size selection: 376-412 bp and 325-425 bp for higher and lower concentration preps, respectively.
University of Michigan