April 2019
Authors:
Benoît Castandet, Arnaud Germain, Amber M. Hotto, and David B. Stern
Info:
Researchers present a protocol called Terminome-Seq that they developed to catalog RNA transcript termini in chloroplast DNA of Arabidopsis thaliana. RNA-seq libraries are prepared for the simultaneous analysis of primary and processed 5’ ends, and 3’ends. The authors indicate that the method helps define promoter sequences, regulatory untranslated regions, and the potential for sense-antisense pairing.
Pippin Prep was used to remove products below 200 bp.
Author Affiliations:
Boyce Thompson Institute, Ithica NY
Cornell University, Ithaca,
Université Paris-Saclay, France
Citation:
BioRxiv
DOI: 10.1101/621938