Comparison of RNA isolation and library preparation methods for small RNA sequencing of canine biofluids

Candice P. Chu, Mary B. Nabity

From the Department of Veterinary Pathobiology at Texas A&M, this study evaluates best practices for small RNA sequencing from canine biofluid samples (serum and urine). The study compares RNA-seq results after using three commercial RNA extraction kits for each biofluid type, and two sequencing library construction kits. A recommendation is provided for isolation/construction for each biofluid, with supporting data.

The PippinHT was used to isolate the small RNA libraries prior to sequencing.

Veterinary Clinical Pathology
DOI: 10.1111/vcp.12743

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