Comprehensive profiling of the vaginal microbiome in HIV positive women using massive parallel semiconductor sequencing

March 2014

Authors:
Adam Ameur, Tracy L. Meiring, Ignas Bunikis, Susana Ha¨ggqvist, Cecilia Lindau, Julia Hedlund Lindberg, Inger Gustavsson, Zizipho Z. A. Mbulawa, Anna-Lise Williamson and Ulf Gyllensten

Info:
Researchers at Uppsala University, Sweden, along with the University of Cape Town and the National Health Laboratory in South Africa, examined co-infections in 20 HIV positive women with a rapid detection sequencing method using rolling circle amplification and the Ion Proton platform. The authors indicate a more comprehensive detection when compared to existing genotyping methods, with the added benefit genomic information being collected. The BluePippin was used to size select samples for Proton template generation.

Citation:
Scientific Reports 4:4398

doi:10.1038/srep04398

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