Author Archives: Alex

Identifying the true number of specimens of the extinct blue antelope (Hippotragus leucophaeus)

Posted on January 15, 2021 by Alex

Jan 2021 Authors: Elisabeth Hempel, Faysal Bibi, J. Tyler Faith, James S. Brink, Daniela C. Kalthoff, Pepijn Kamminga, Johanna L. A. Paijmans, Michael V. Westbury, Michael Hofreiter & Frank E. Zachos Info: In an effort to better understand the mechanisms … more »

Posted in Citation | Tagged | Comments Off on Identifying the true number of specimens of the extinct blue antelope (Hippotragus leucophaeus)

ATAC-seq with unique molecular identifiers improves quantification and footprinting

Posted on November 19, 2020 by Alex

November 2020 Authors: Tao Zhu, Keyan Liao, Rongfang Zhou, Chunjiao Xia & Weibo Xie Info: The authors suggest an improvement on the ATAC-seq method (Assay for Transposase-Accessible Chromatin with high-throughput sequencing). By using unique molecular identifiers to distinguish between transposase … more »

Posted in Citation | Tagged , | Comments Off on ATAC-seq with unique molecular identifiers improves quantification and footprinting

HLS-CATCH and TELL-Seq: Haplotype Phasing of a 187kb HMW Gene Targets from a Trio

Posted on November 11, 2020 by Alex

We here at Sage Science are excited to have joined forces with Universal Sequencing Technology to promote their TELL-Seq barcode linked-read technology.  One of the reasons for our excitement is that TELL-Seq, and the transposase-based barcoding method benefits greatly from … more »

Posted in Blog | Tagged , | Comments Off on HLS-CATCH and TELL-Seq: Haplotype Phasing of a 187kb HMW Gene Targets from a Trio

Complete and haplotype-specific sequence assembly of segmental duplication-mediated genome rearrangements using targeted CRISPR-targeted ultra-long read sequencing (CTLR-Seq)

Posted on October 30, 2020 by Alex

October 2020 Authors: Bo Zhou, GiWon Shin, Stephanie U. Greer, Lisanne Vervoort, Yiling Huang, Reenal Pattni, Marcus Ho, Wing H. Wong, Joris R. Vermeesch, Hanlee P. Ji, Alexander E. Urban Info: In this preprint the authors report on a method, … more »

Posted in Citation | Tagged , , , | Comments Off on Complete and haplotype-specific sequence assembly of segmental duplication-mediated genome rearrangements using targeted CRISPR-targeted ultra-long read sequencing (CTLR-Seq)

Ultra-low input single tube linked-read library method enables short-read second-generation sequencing systems to generate highly accurate and economical long-range sequencing information routinely generate highly accurate and economical long-range sequencing information

Posted on October 27, 2020 by Alex

June 2020 Authors: Zhoutao Chen, Long Pham, Tsai-Chin Wu, Guoya Mo, Yu Xia, Peter L Chang, Devin Porter, Tan Phan, Huu Che, Hao Tran, Vikas Bansal, Justin Shaffer, Pedro Belda-Ferre, Gregory Humphrey, Rob Knight, Pavel Pevzner, Son Pham, Yong Wang … more »

Posted in Citation | Tagged | Comments Off on Ultra-low input single tube linked-read library method enables short-read second-generation sequencing systems to generate highly accurate and economical long-range sequencing information routinely generate highly accurate and economical long-range sequencing information

CRISPR–Cas9/long-read sequencing approach to identify cryptic mutations in BRCA1 and other tumour suppressor genes

Posted on October 27, 2020 by Alex

October 2020 Authors: Tom Walsh, Silvia Casadei, Katherine M Munson, Mary Eng, Jessica B Mandell, Suleyman Gulsuner, Mary-Claire King Info: In this short report, researchers studying young-onset breast cancer used the SageHLS and HLS-CATCH method to purify high molecular weight … more »

Posted in Citation | Tagged , , , | Comments Off on CRISPR–Cas9/long-read sequencing approach to identify cryptic mutations in BRCA1 and other tumour suppressor genes

Sage Science to Distribute Universal Sequencing Technology’s TELL-Seq™ Linked-Read NGS Library Prep Kits

Posted on October 26, 2020 by Alex

BEVERLY, Mass., and CANTON, Mass. — October 26, 2020 — Sage Science, a developer of products for improving sample preparation processes in life science applications, and Universal Sequencing Technology today announced a distribution agreement to improve scientists’ access to long-range … more »

Posted in News, News, Press Release | Comments Off on Sage Science to Distribute Universal Sequencing Technology’s TELL-Seq™ Linked-Read NGS Library Prep Kits

Unleash Your Illumina Sequencer with TELL-Seq™ Linked Reads

Posted on October 26, 2020 by Alex

We’re proud to announce today that we will be selling TELL-Seq™ linked library prep kits from Universal Sequencing Technology. TELL-Seq, Transposase Enzyme Linked Long-read Sequencing, is a scalable NGS library technology with a very simple workflow that can provide you … more »

Posted in Applications, Blog | Comments Off on Unleash Your Illumina Sequencer with TELL-Seq™ Linked Reads

A long-read RNA-seq approach to identify novel transcripts of very large genes

Posted on July 14, 2020 by Alex

July 2020 Authors: Prech Uapinyoying, Jeremy Goecks, Susan M. Knoblach, Karuna Panchapakesan, Carsten G. Bonnemann, Terence A. Partridge, Jyoti K. Jaiswal, and Eric P. Hoffman Info: Scientists researching muscular disorders present a method for studying gene expression of large RNA … more »

Posted in Citation | Tagged , , | Comments Off on A long-read RNA-seq approach to identify novel transcripts of very large genes

Fully phased sequence of a diploid human genome determined de novo from the DNA of a single individual

Posted on July 14, 2020 by Alex

July 2020 Authors: Ilya Soifer , Nicole L. Fong, Nelda Yi, Andrea T. Ireland, Irene Lam, Matthew Sooknah, Jonathan S. Paw, Paul Peluso, Gregory T. Concepcion, David Rank, Alex R. Hastie, 5 Vladimir Jojic, J. Graham Ruby, David Botstein, Margaret … more »

Posted in Citation | Tagged , , | Comments Off on Fully phased sequence of a diploid human genome determined de novo from the DNA of a single individual