Gene by environment interaction mouse model reveals a functional role for 5-hydroxymethylcytosine in neurodevelopmental disorders

May 2021

Ligia A. Papale, Andy Madrid, Qi Zhang, Kailei Chen, Lara Sak, Sündüz Keles, and Reid S. Alisch

Researchers studying the role of 5-Hydroxymethylcytosine (5hmC) epigenetic modifications in neurological disorders report on the effect of prenatal stress on 5hMC abundance in mice. Knockouts of the Cntnap2 gene in mice have shown disruption in 5hmC, resulting in behavioral disorders. The researchers subjected mice that were heterozygous for Cntnap2, which are behaviorally normal, to prenatal stress. As adults these mice exhibited similar abnormalities. Using NGS and ChIP-seq, the authors propose a mechanism by which 5hmC alters the binding of the transcription factor CLOCK near the promoters of several genes.

PippinHT size select ChIP libraries (~400 bp) generated by the NEBNext® ChIP-Seq Library Prep Kit.

Author Affiliations:
Department of Neurological Surgery,
Department of Statistics, Biostatistics, and Medical Informatics,
Department of Neuroscience training program, University of Wisconsin, Madison, WI
Department Mathematics and Statistics, University of New Hampshire, Durham, NH

bioRxiv preprint
DOI: 10.1101/2021.05.04.441625

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