September 2012
Authors:
Rachel Sealfon, Stephen Gire, Crystal Ellis, Stephen Calderwood, Firdausi Qadri, Lisa Hensley, Manolis Kellis, Edward T Ryan, Regina C LaRocque, Jason B Harris, Pardis C Sabeti
Info:
Study performs high-depth NGS on V. cholerae strains from Haiti and DR, some of which had been previously typed by less-thorough PacBio and Illumina studies. Bacterial DNA was fragmented by nebularization, size selected tight at 200bp on Pippin Prep, then prepared library (end-repair, add adapters, enrich PCR for 15 cycles) and sequenced on Illumina HiSeq.
Citation:
BMC Genomics 2012, 13:468