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Monthly Archives: April 2013
Improved Recovery for 3-10kb DNA Size Selection
BluePippin users, we have a new cassette definition that will appear in the Cassette Definition Set 10 (CD10) that we feel merits some further detail. This definition is named “0.75% DF 3 – 10kb Marker S1 – Improved Recovery” What … more »
Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
April 2013 Authors: Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson Info: The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification … more »
Posted in Citation
Tagged Amplicon, DNA Sequencing, Featured, Pippin Prep, Pippin System
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At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies
Before a new technology is deployed at The Genome Analysis Centre in Norwich, UK, it must run the gauntlet of Matthew Clark’s lab. Clark is the sequencing technology development leader at TGAC, where part of his role involves testing out … more »
Posted in Blog
Tagged BluePippin, DNA Sequencing, Illumina, Mate-pair Sequencing, PacBio, Pippin System
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Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae
April 2013 Authors: Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen Info: The Pippin Prep was used to size-select Illumina RNA-seq libraries. Citation: Int. J. Mol. Sci. 2013, 14, 9080-9098 http://dx.doi.org/10.3390/ijms14059080
Posted in Citation
Tagged Illumina, Pippin Prep, Pippin System, RNA-seq
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NHGRI Scientists Find Transgenic DNA Has More Rearrangements than Expected
Amanda DuBose didn’t set out to come up with a new technical solution to characterizing transgenic DNA in animal models. A postdoctoral fellow in Francis Collins’ lab at the National Human Genome Research Institute, DuBose was using mice to study … more »
Posted in Blog
Tagged DNA Sequencing, Pippin Prep
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Selective Inhibition of Tumor Oncogenes by Disruption of Super-Enhancers
April 2013 Authors: Jakob Love, Heather A. Hoke, Charles Y. Lin, Ashley Lau, David A. Orlando, Christopher R. Vakoc, James E. Bradner, Tong Ihn Lee, and Richard A. Young Info: The Pippin Prep was used to size-select ChIP-seq libraries. Citation: … more »
Posted in Citation
Tagged ChIP-Seq, DNA Sequencing
Comments Off on Selective Inhibition of Tumor Oncogenes by Disruption of Super-Enhancers