Monthly Archives: November 2012

And Now, a Word from Our Customer Service Department

Posted on November 28, 2012 by Alex

Here at Sage Science, we are delighted to see more and more people signing up as customers of the Pippin platform. With so many instruments out in the wild, we thought it would be a good time to sit down … more »

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SATB1-mediated functional packaging of chromatin into loops

Posted on November 23, 2012 by Alex

November 2012 Authors: Terumi Kohwi-Shigematsu, Yoshinori Kohwi, Keiko Takahashi, Hunter W. Richards, Stephen D. Ayers, Hye-Jung Han, Shutao Cai Info: Among other things, this paper outlines methods for mapping all DNA binding sites for a global chromatin organizing protein, SATB1. … more »

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For Low-Input DNA Work, Scientists Recommend Pippin for Best Size Selection

Posted on November 20, 2012 by Alex

Scientists at the University of Arizona, led by senior author and newly named Moore Foundation Investigator Matthew Sullivan, have published details of how to optimize sample prep methods for next-gen sequencing projects in which input DNA is negligible. As part … more »

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Use of Four Next-Generation Sequencing Platforms to Determine HIV-1 Coreceptor Tropism

Posted on November 18, 2012 by Alex

November 2012 Authors: John Archer, Jan Weber, Kenneth Henry, Dane Winner, Richard Gibson, Lawrence Lee, Ellen Paxinos, Eric J. Arts, David L. Robertson, Larry Mimms, Miguel E. Quinones-Mateu Info: Comparative study of four NGS platforms (454, Illumina, PacBio, Ion) in … more »

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Next-Generation Sequencing for Rodent Barcoding: Species Identification from Fresh, Degraded and Environmental Samples

Posted on November 16, 2012 by Alex

November 2012 Authors: Maxime Galan, Marie Pages, Jean-Francois Cosson Info: Genotyping rodent strains using targeted NGS of informative regions of cytochrome b and cytochrome c oxidase 1 genes. PCR fragments ~250 bp in length were generated (with barcoded primers) from … more »

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Crop Science Collaboration Uses Next-Gen Sequencing with Pippin Prep

Posted on November 12, 2012 by Alex

A collaboration among scientists at Monsanto and Bayer Crop Science has demonstrated the use of next-generation sequencing and a new bioinformatics method for analyzing the genome sequence of crops that have been genetically modified. Lead authors David Kovalic and Carl … more »

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At ASHG, Interest Soars for Advances in Sequencing Technologies

Posted on November 9, 2012 by Alex

We were thrilled to escape the apocalyptic weather of the northeast for a few days to attend the American Society of Human Genetics annual meeting in San Francisco. It’s one of our favorite meetings, and with nearly 7,000 scientists registered, … more »

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Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation

Posted on November 1, 2012 by Alex

November 2012 Authors: Michael A. Quail, Yong Gu, Harold Swerdlow, Matthew Mayho Info: An evaluation of semi-automated size selection systems for use in size-selection for NGS libraries at the Sanger Centre. A comparison to manual gel purification and magnetic beads. … more »

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