Tag Archives: RAD-seq

RAD-seq, High-Pass Sizing in Sunny San Diego

Posted on January 13, 2017 by Alex

We are shaking off this northeast winter and heading out to San Diego for the 25th annual International Plant and Animal Genome meeting! This event is always a great way to kick off the year, surrounded by cutting-edge science and … more »

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Double Pippin for Optimized RAD-seq

Posted on July 13, 2016 by Alex

Scientists in China and the UK recently published an open-access optimized protocol for RAD-seq in the Theoretical and Applied Genetics journal. The method is targeted at large studies of plants and enables users to specify sequence coverage parameters. From lead … more »

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A highly robust and optimized sequence-based approach for genetic polymorphism discovery and genotyping in large plant populations

Posted on June 13, 2016 by Alex

May 2016 Authors: Ning Jiang, Fengjun Zhang, Jinhua Wu, Yue Chen, Xiaohua Hu, Ou Fang, Lindsey J. Leach, Di Wang, Zewei Luo Info: Scientists in China and the UK developed an optimized RAD-seq protocol that delivers better uniformity of genome … more »

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New hyRAD Method Expands Use of RAD-seq to Degraded Samples

Posted on March 31, 2016 by Alex

Since RAD-seq was first developed, we’ve seen a number of new versions and approaches from an enthusiastic scientific community. The latest was recently published in PLoS One and demonstrates a RAD-based method suitable for analyzing degraded DNA, an essential step … more »

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Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens

Posted on March 31, 2016 by Alex

March 2016 Authors: Tomasz Suchan, Camille Pitteloud, Nadezhda S. Gerasimova, Anna Kostikova, Sarah Schmid, Nils Arrigo, Mila Pajkovic, Michał Ronikier, and Nadir Alvarez Info: Scientists present a new protocol based on the RAD-seq concept that works more effectively for samples … more »

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Genome-wide SNPs lead to strong signals of geographic structure and relatedness paterns in the major arbovirus vector, Aedes aegypti

Posted on April 7, 2014 by Alex

April 2014 Authors: Gordana Rašić, Igor Filipović, Andrew R Weeks and Ary A Hoffmann Info: From the Pest and Disease Vector Group, Department of Genetics, the University of Melbourne Australia. The researchers developed a new bioinformatics tool for the ddRAD … more »

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Genomic architecture of ecologically divergent body shape in a pair of sympatric crater lake cichlid fishes

Posted on October 6, 2013 by Alex

October 2013 Authors: PAOLO FRANCHINI, CARMELO FRUCIANO, MARIA L. SPREITZER, JULIA C. JONES, KATHRYN R. ELMER, FREDERICO HENNING and AXEL MEYER Info: Researchers at the University of Konstanz, Germany, look at the speciation of chichlid fishes using the ddRAD technique. … more »

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Discrete genetic modules are responsible for complex burrow evolution in Peromyscus mice

Posted on January 9, 2013 by Alex

January 2013 Authors: Jesse N. Weber, Brant K. Peterson & Hopi E. Hoekstra Info: The work seeks to identify genetic influences on a complex behavior in mice, burrow formation. The authors used a modified RADseq procedure to genotype two species … more »

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A Hybrid Genetic Linkage Map of Two Ecologically and Morphologically Divergent Midas Cichlid Fishes(Amphilophus spp.) Obtained by Massively Parallel DNA Sequencing (ddRADSeq)

Posted on January 1, 2013 by Alex

January 2013 Authors: Hans Recknagel, Kathryn R. Elmer, and Axel Meyer Info: RADseq was used to develop linkage maps for two highly divergent Cichlid fish. Pippin Prep was used to size select the libraries. Citation: G3 (Bethesda) 3(1):65-74 January 2013 … more »

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Holiday Greetings from Sage Science

Posted on December 28, 2012 by Alex

It’s that time of year again — we slow down at work, do some reflection, and gear up for a great (lucky?) ‘13…. In the field of genomics, 2012 was a fascinating year. On the NGS front, placements of desktop … more »

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