Tag Archives: Pippin Prep

At Iowa Core Lab, Pippin Sizing Is Essential for MicroRNA Studies

Posted on March 21, 2013 by Alex

Kevin Knudtson’s DNA Facility at the University of Iowa acquired a Pippin Prep almost a year ago, and in the time since, the automated size selection tool has become an integral part of the core lab’s microRNA pipeline. “Pippin is … more »

Posted in Blog | Tagged , , , | Comments Off on At Iowa Core Lab, Pippin Sizing Is Essential for MicroRNA Studies

Pippin Shines in Size Selection Comparison from Sanger

Posted on March 13, 2013 by Alex

A paper that came out December in the journal Electrophoresis compares Pippin Prep size selection with Caliper’s Labchip XT and finds that Pippin gives “very tight size distributions” not seen with other sizing methods. “Evaluation and optimisation of preparative semi-automated … more »

Posted in Blog | Tagged , , | Comments Off on Pippin Shines in Size Selection Comparison from Sanger

At MIT, Pippin Enables Splice Variant Detection and MicroRNA Analysis

Posted on February 26, 2013 by Alex

At the BioMicro Center at MIT, Director Stuart Levine, PhD, recently introduced the Pippin Prep from Sage Science to enable key applications — including splice variant analysis with RNA-seq and microRNA analysis — that were not possible on other platforms. … more »

Posted in Blog | Tagged , , | Comments Off on At MIT, Pippin Enables Splice Variant Detection and MicroRNA Analysis

Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential

Posted on February 1, 2013 by Alex

January/February 2013 Authors: Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana Info: Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome … more »

Posted in Citation | Tagged , , | Comments Off on Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential

Pippin Platform Recommended for Nextera Mate Pair Size Selection

Posted on January 24, 2013 by Alex

Illumina released new sample prep protocol guidelines for generating mate pair libraries with its Nextera kit, and we’re pleased to report that the Pippin platform is the recommended choice for automated size selection. You can check out the Nextera Mate … more »

Posted in Blog | Tagged , , | Comments Off on Pippin Platform Recommended for Nextera Mate Pair Size Selection

A genome-wide survey of genetic variation in gorillas using reduced representation sequencing

Posted on January 23, 2013 by Alex

January 2013 Authors: Aylwyn Scally, Bryndis Yngvadottir, Yali Xue, Qasim Ayub, Richard Durbin, Chris Tyler-Smith Info: The study used reduced representation libraries to study diversity among the two currently recognized species of gorilla. The libraries were generated by AluI digestion … more »

Posted in Citation | Tagged , | Comments Off on A genome-wide survey of genetic variation in gorillas using reduced representation sequencing

Poster: Exome Sequencing with Ion Torrent and Pippin Prep

Posted on January 23, 2013 by Alex

In a poster from Ion Torrent (Life Technologies) for 2012’s ASHG, scientists looked at exome sequencing by studying a familial trio on both the PGM™ and the Proton™ instruments. Size selection for both sequencers was performed on the Pippin Prep … more »

Posted in Blog | Tagged , , | Comments Off on Poster: Exome Sequencing with Ion Torrent and Pippin Prep

Tag Team: At DFCI, Pippin and Nextera Make Better Libraries

Posted on January 18, 2013 by Alex

Over at the Dana-Farber Cancer Institute, scientists have been doing some really interesting work pairing the Pippin Prep with Illumina’s Nextera kit for library preparation. Zach Herbert, associate director of the Molecular Biology Core Facilites at DFCI, says that he … more »

Posted in Blog | Tagged , , , | Comments Off on Tag Team: At DFCI, Pippin and Nextera Make Better Libraries

DNA for Species Identification: A Study of Rodents

Posted on January 18, 2013 by Alex

Carl Linnaeus would be proud: A recent paper in PLoS One demonstrates the use of next-gen sequencing with genetic barcodes to accurately identify more than 100 different species from the Rodentia order. In the study, amplicons were run on the … more »

Posted in Blog | Tagged , | Comments Off on DNA for Species Identification: A Study of Rodents

Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene

Posted on January 11, 2013 by Alex

January 2013 Authors: Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins Info: The authors designed a general method to study the arrangement of foreign DNA and host … more »

Posted in Citation | Tagged , , , | Comments Off on Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene