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Tag Archives: Pippin Prep
Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential
January/February 2013 Authors: Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana Info: Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep
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Pippin Platform Recommended for Nextera Mate Pair Size Selection
Illumina released new sample prep protocol guidelines for generating mate pair libraries with its Nextera kit, and we’re pleased to report that the Pippin platform is the recommended choice for automated size selection. You can check out the Nextera Mate … more »
Posted in Blog
Tagged Illumina, Mate-pair Sequencing, Pippin Prep
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A genome-wide survey of genetic variation in gorillas using reduced representation sequencing
January 2013 Authors: Aylwyn Scally, Bryndis Yngvadottir, Yali Xue, Qasim Ayub, Richard Durbin, Chris Tyler-Smith Info: The study used reduced representation libraries to study diversity among the two currently recognized species of gorilla. The libraries were generated by AluI digestion … more »
Posted in Citation
Tagged DNA Sequencing, Pippin Prep
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Poster: Exome Sequencing with Ion Torrent and Pippin Prep
In a poster from Ion Torrent (Life Technologies) for 2012’s ASHG, scientists looked at exome sequencing by studying a familial trio on both the PGM™ and the Proton™ instruments. Size selection for both sequencers was performed on the Pippin Prep … more »
Posted in Blog
Tagged DNA Sequencing, Ion Torrent, Pippin Prep
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Tag Team: At DFCI, Pippin and Nextera Make Better Libraries
Over at the Dana-Farber Cancer Institute, scientists have been doing some really interesting work pairing the Pippin Prep with Illumina’s Nextera kit for library preparation. Zach Herbert, associate director of the Molecular Biology Core Facilites at DFCI, says that he … more »
Posted in Blog
Tagged Amplicon, DNA Sequencing, Illumina, Pippin Prep
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DNA for Species Identification: A Study of Rodents
Carl Linnaeus would be proud: A recent paper in PLoS One demonstrates the use of next-gen sequencing with genetic barcodes to accurately identify more than 100 different species from the Rodentia order. In the study, amplicons were run on the … more »
Posted in Blog
Tagged DNA Sequencing, Pippin Prep
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Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene
January 2013 Authors: Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins Info: The authors designed a general method to study the arrangement of foreign DNA and host … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Paired-end Sequencing, Pippin Prep
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A Hybrid Genetic Linkage Map of Two Ecologically and Morphologically Divergent Midas Cichlid Fishes(Amphilophus spp.) Obtained by Massively Parallel DNA Sequencing (ddRADSeq)
January 2013 Authors: Hans Recknagel, Kathryn R. Elmer, and Axel Meyer Info: RADseq was used to develop linkage maps for two highly divergent Cichlid fish. Pippin Prep was used to size select the libraries. Citation: G3 (Bethesda) 3(1):65-74 January 2013 … more »
Posted in Citation
Tagged DNA Sequencing, Pippin Prep, RAD-seq
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Holiday Greetings from Sage Science
It’s that time of year again — we slow down at work, do some reflection, and gear up for a great (lucky?) ‘13…. In the field of genomics, 2012 was a fascinating year. On the NGS front, placements of desktop … more »
Posted in Blog
Tagged BluePippin, DNA Sequencing, Pippin Prep, RAD-seq
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IL-10 Produced by Induced Regulatory T Cells (iTregs) Controls Colitis and Pathogenic Ex-iTregs during Immunotherapy
December 2012 Authors: Erica G. Schmitt, Dipica Haribhai, Jason B. Williams, Praful Aggarwal, Shuang Jia, Louis-Marie Charbonnier, Ke Yan, Rachel Lorier, et al. Info: The study included sequencing of the T-cell receptor. For this sequencing, the authors used Ion Torrent … more »
Posted in Citation
Tagged DNA Sequencing, Ion Torrent, Pippin Prep
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