Tag Archives: PippinHT

For 10x Genomics Workflow, Broad Institute Uses PippinHT Size Selection

Posted on July 18, 2017 by Alex

At the Broad Institute, scientist Michelle Cipicchio is part of the technology development team responsible for optimizing new methods or sample types before they’re implemented on the organization’s industrial-scale exome and whole-genome sequencing pipeline. Recently, she’s been working with the … more »

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PacBio Users: Size Selection Is Essential for Generating Excellent Results

Posted on July 6, 2017 by Alex

The Sage Science team was delighted to attend and co-sponsor PacBio’s annual East Coast user group meeting in Baltimore last week, particularly since there was a half-day session devoted to our favorite subject: sample prep. There were plenty of customer … more »

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Preprint Highlights Utility of Size Selection for Nanopore Studies

Posted on June 29, 2017 by Alex

A recently shared preprint demonstrates the effectiveness of size-selection for nanopore sequencing, relying on the PippinHT automated DNA sizing platform for high-throughput pipelines. “Mapping And Phasing Of Structural Variation In Patient Genomes Using Nanopore Sequencing” comes from lead author Mircea … more »

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Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication

Posted on April 25, 2017 by Alex

March 2017 Authors: Maojun Wang, Lili Tu, Min Lin, Zhongxu Lin, et al. Info: Scientists from China and the UK scanned the genomes of 352 varieties of cotton, both wild and domestic, to map variants associated with crop domestication throughout … more »

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MicroRNA biomarkers of pancreatic injury in a canine model

Posted on February 25, 2017 by Alex

January 2017 Authors: Rodney Rouse, Barry Rosenzweig, Katie Shea, Alan Knapton, Sharron Stewart, Lin Xu, Ashok Chockalingam, Leah Zadrozny, and Karol Thompson Info: FDA scientists report results of studying five pancreas-enriched microRNAs as potential biomarkers for pancreatic injury. They used … more »

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Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining

Posted on January 6, 2017 by Alex

December 2016 Authors: Peter Ly, Levi S. Teitz, Dong H. Kim, Ofer Shoshani, Helen Skaletsky, Daniele Fachinetti, David C. Page, and Don W. Cleveland Info: Scientists report developing a strategy to examine what happens when chromosomes do not segregate properly. … more »

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Low-Coverage Sequencing No Challenge for Structural Variant Detection

Posted on July 21, 2016 by Alex

Is it really possible to detect somatic structural variants accurately from a single sequencing read? A new protocol from scientists at the Albert Einstein College of Medicine in New York and Voronezh State University in Russia was designed to do … more »

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Quantitative detection of low-abundance somatic structural variants in normal cells by high-throughput sequencing

Posted on July 21, 2016 by Alex

June 2016 Authors: Wilber Quispe-Tintaya, Tatyana Gorbacheva, Moonsook Lee, Sergei Makhortov, Vasily N Popov, Jan Vijg & Alexander Y Maslov Info: Scientists report Structural Variant Search, a new method for detecting rare somatic structural variants even with low-coverage sequencing data. … more »

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Sage Sizing Platforms Recommended for 10x Genomics Libraries

Posted on July 18, 2016 by Alex

We’re pleased to report that 10x Genomics has released a new sample prep protocol for its Chromium platform that includes the BluePippin and PippinHT size selection platforms from Sage Science. 10x Genomics has gotten a lot of attention in recent … more »

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Spotted in the Literature: The First PippinHT Publication!

Posted on May 9, 2016 by Alex

We began shipping the high-throughput version of our automated Pippin DNA size selection platform last year, and it’s a thrill to see what we believe is the first reference to it in a peer-reviewed publication. A team of scientists from … more »

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