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Tag Archives: PacBio
A survey of the sorghum transcriptome using single-molecule long reads
June 2016 Authors: Salah E. Abdel-Ghany, Michael Hamilton, Jennifer L. Jacobi, Peter Ngam, Nicholas Devitt, Faye Schilkey, Asa Ben-Hur & Anireddy S. N. Reddy Info: In this publication, scientists from Colorado State University and the National Center for Genome Resources … more »
Posted in Citation
Tagged BluePippin, isoseq, PacBio, RNAseq, transcriptome
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Scientists Use Multiple Technologies to Produce High-Quality Chinese Genome Assembly
A newly reported genome assembly of a Chinese individual, generated by scientists in China and the US, used long-read PacBio sequencing, short-read Illumina data, and BioNano Genomics physical maps to achieve remarkably high accuracy and contiguity. Along the way, the … more »
Posted in Blog
Tagged BluePippin, PacBio
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Long-read sequencing and de novo assembly of a Chinese genome
June 2016 Authors: Lingling Shi, Yunfei Guo, Chengliang Dong, John Huddleston, Hui Yang, et al. Info: Scientists in China and the US analyzed the DNA and RNA of a Chinese individual, using PacBio sequencing and BioNano Genomics maps to produce … more »
Posted in Citation
Tagged BluePippin, PacBio
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Extensive sequencing of seven human genomes to characterize benchmark reference materials
June 2016 Authors: Justin M. Zook, David Catoe, Jennifer McDaniel, Lindsay Vang, et al. Info: The Genome in a Bottle Consortium used 12 different DNA analysis technologies to generate deep data sets on seven genomes, five of which will become … more »
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Tagged BluePippin, PacBio
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NCSU Scientist Incorporates Genomics for Better Blueberry Resources
Hamid Ashrafi is working to breed higher-quality blueberries that are amenable to mechanical harvest, larger in size, tastier, and have a longer shelf life. As an assistant professor at North Carolina State University, Ashrafi is bringing genomic tools to a … more »
Posted in Blog
Tagged BluePippin, isoseq, PacBio, SageELF
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The Conference Month: Prepping for PacBio, ASM, FoG
It’s June, and you know what that means: genomics conference season is back! The Sage team will be attending several events this month and we hope to see you at least once. We kick off next week with PacBio’s annual … more »
For PacBio’s Iso-Seq Method, Users Deploy SageELF or BluePippin Size Selection
More and more scientists are using their PacBio systems for transcriptome studies, generating full-length isoforms with the Iso-Seq method. The number of novel transcripts discovered and the implications for alternative splicing are a not-so-subtle reminder that we still have a … more »
ChIA-PET Study Demonstrates Effect of Genome Folding on Gene Expression
For a recent publication in Cell, scientists used the SageELF whole-sample fractionation platform to perform size selection prior to cDNA sequencing on the PacBio system. The Iso-Seq method allows PacBio users to generate full-length transcripts, and SageELF makes it easier … more »
Long Reads in All Their Glory: PacBio, ONT User Meetings Report Coming Advances
A pair of user group meetings last week offered some intriguing glimpses into the future of long-read sequencing. Oxford Nanopore customers got together in New York, while PacBio users assembled in Palo Alto, Calif. The Sage Science team attended and … more »
Posted in Blog
Tagged BluePippin, long reads, ONT, PacBio
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Scarless genome editing and stable inducible expression vectors for Geobacter sulfurreducens
August 2015 Authors: Chi Ho Chan, Caleb E. Levar, Lori Zacharoff, Jonathan P. Badalamenti, Daniel R. Bond Info: Scientists from the University of Minnesota report in Applied and Environmental Microbiology the development of genetic system improvements for Geobacter sulfurreducens. BluePippin … more »
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Tagged BluePippin, PacBio
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