Tag Archives: isoseq

Iso-Seq Allows Genome-Independent Transcriptome Profiling of Grape Berry Development

Posted on March 19, 2019 by Alex

March 2019 Authors: Andrea Minio, Mélanie Massonnet, Rosa Figueroa-Balderas, Amanda M. Vondras, Barbara Blanco-Ulate, and Dario Cantu Info: Researchers used PacBio’s Iso-Seq method to sequence full-length cDNA transcripts from ripening Cabernet Savignon berries resulting in 1,5000 cultivar-specific genes. The authors … more »

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A survey of the sorghum transcriptome using single-molecule long reads

Posted on July 31, 2016 by Alex

June 2016 Authors: Salah E. Abdel-Ghany, Michael Hamilton, Jennifer L. Jacobi, Peter Ngam, Nicholas Devitt, Faye Schilkey, Asa Ben-Hur & Anireddy S. N. Reddy Info: In this publication, scientists from Colorado State University and the National Center for Genome Resources … more »

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NCSU Scientist Incorporates Genomics for Better Blueberry Resources

Posted on June 21, 2016 by Alex

Hamid Ashrafi is working to breed higher-quality blueberries that are amenable to mechanical harvest, larger in size, tastier, and have a longer shelf life. As an assistant professor at North Carolina State University, Ashrafi is bringing genomic tools to a … more »

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For PacBio’s Iso-Seq Method, Users Deploy SageELF or BluePippin Size Selection

Posted on May 24, 2016 by Alex

More and more scientists are using their PacBio systems for transcriptome studies, generating full-length isoforms with the Iso-Seq method. The number of novel transcripts discovered and the implications for alternative splicing are a not-so-subtle reminder that we still have a … more »

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ChIA-PET Study Demonstrates Effect of Genome Folding on Gene Expression

Posted on January 19, 2016 by Alex

For a recent publication in Cell, scientists used the SageELF whole-sample fractionation platform to perform size selection prior to cDNA sequencing on the PacBio system. The Iso-Seq method allows PacBio users to generate full-length transcripts, and SageELF makes it easier … more »

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