Tag Archives: Illumina

Next-Generation Sequence Analysis of Cancer Xenograft Models

Authors: Fernando J. Rossello, Richard W. Tothill, Kara Britt, Kieren D. Marini, Jeanette Falzon, David M. Thomas, Craig D. Peacock, Luigi Marchionni, Jason Li, Samara Bennett, Erwin Tantoso, Tracey Brown, Philip Chan, Luciano G. Martelotto, D. Neil Watkins Info: The … more »

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In Skin Microbiome Study, Scientists Find Host Genotype Influences Bacterial Populations

A paper that came out in the Journal of Innate Immunity this summer reports that a person’s genotype affects which microbes will colonize his or her skin, which in turn may alter that person’s defense mechanisms against pathogenic organisms. “Skin … more »

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De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana

August 2013 Authors: Stephen M Gross, Jeffrey A Martin, June Simpson, María Jazmín Abraham-Juarez, Zhong Wang and Axel Visel Info: Agave is an interesting plant for bioenergy study. This paper provides transcriptome data of two strains. The Pippin Prep was … more »

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In Sanger Study, BluePippin Contributes to Improved Construction Method for Mate-Pair Libraries

Scientists from the Wellcome Trust Sanger Institute recently published “An improved approach to mate-paired library preparation for Illumina sequencing,” a paper that came out in the open access journal Methods in Next Generation Sequencing. The publication, from lead author Naomi … more »

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Comparative genomics of Campylobacter concisus isolates reveals genetic diversity and provides insights into disease association

August 2013 Authors: Nandan P Deshpande, Nadeem O Kaakoush, Marc R Wilkins and Hazel M Mitchell Info: Collaborators at the University of New South Wales sequence several strains of Campylobacter concisus from healthy and diseased (Crohn’s disease and gastroenteritis) individuals … more »

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A quantitative reference transcriptome for Nematostella vectensis early embryonic development: a pipeline for de novo assembly in emerging model systems

June 2013 Authors: Sarah Tulin, Derek Aguiar, Sorin Istrail, and Joel Smith Info: Scientists from the Marine Biological Laboratory and Brown University developed a new pipeline for de novo transcriptome assembly that can be used with organisms lacking a well-annotated … more »

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Phylogeographic Origin of Helicobacter pylori Determines Host-Adaptive Responses upon Coculture with Gastric Epithelial Cells

July 2013 Authors: Alexander Sheh, Rupesh Chaturvedi, D. Scott Merrell, Pelayo Correa, Keith T. Wilson, James G. Fox Info: A study of gene expression comparing several strains of H. pylori. The BluePippin was used to size-select fragments to construct paired-end … more »

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Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses

Authors: Sanne P. Smeekens, c Curtis Huttenhower, Anca Riza, Frank L. van de Veerdonk, Patrick L.J.M. Zeeuwen, Joost Schalkwijk, Jos W.M. van der Meer, Ramnik J. Xavier, Mihai G. Netea, Dirk Gevers Info: Genomic DNA was subjected to 16S PCR … more »

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A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology

June 2013 Authors: A. Murat Eren, Joseph H. Vineis., Hilary G. Morrison, Mitchell L. Sogin Info: The authors present a method of library preparation for overlapping paired-end sequencing on the Illumina platform, and improved analysis software, to reduce the error … more »

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Deciphering the Growth Behaviour of Mycobacterium africanum

May 2013 Authors: Florian Gehre, Jacob Otu, Kathryn DeRiemer, Paola Florez de Sessions, Martin L. Hibberd, Wim Mulders, Tumani Corrah, Bouke C. de Jong, Martin Antonio Info: The Pippin Prep was used to size select libraries prepared with the New … more »

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